Mnasicles (Remella) ecua Grishin, 2023

Zhang, Jing, Cong, Qian & Grishin, Nick V., 2023, Supplementary Materials and Appendix, Insecta Mundi 2023 (26), pp. 1-115 : 57-58

Mnasicles (Remella) ecua Grishin , new species

https://zoobank.org/ E5849270-FF1F-4A4A-B099-B9097FABABDF

( Fig. 6 part, 135–136, 366–367)

Definition and diagnosis. Phylogenetic trees reveal that a specimen from Ecuador identified as Mnasicles (Remella) remus (Fabricius, 1798) (type locality in “Caiena”) shows prominent genetic differentiation from it ( Fig. 6): e.g., their COI barcodes differ by 4.6% (30 bp), and therefore represents a new species. This new species keys to “ Moeris remus ” (J.33.1) in Evans (1955) but differs from it by more developed pale area at the apex of ventral forewing ( Fig. 136), not strong spots as in Mnasicles (Remella) rita ( Evans, 1955) (type locality in Guatemala) and relatives, but more prominent than in most M. remus , uncus smaller, terminally knob-like, strongly humped in lateral view, tegumen short, not much longer than vinculum width, harpe shorter, with less developed tooth on the dorsal side, costa convex in the middle and slightly concave before ampulla that is separated from harpe by a narrower groove ( Fig. 366–367). Due to the cryptic nature of this species, most reliable identification is achieved by DNA and a combination of the following base pairs is diagnostic in the nuclear genome: aly216.58.1:T366A, aly216.58.1:A492C, aly 1456.2.1:T15C, aly1139.65.24:T108A, aly1139.65.24:A117C, aly 2954.3.1:T525T (not A), aly 2954.3.1:C800C (not T), aly127.87.1:T24T (not G), aly127.87.1:A177A (not G), aly11700.1.14:A59A (not T), and COI barcode: T5T, A37G, A100G, T316G, T463C.

Barcode sequence of the holotype. Sample NVG-19017B02, GenBank OR837685, 658 base pairs: AACTTTATATTTTATTTTTGGAATTTGAGCAGGAATATTAGGAACTTCTTTAAGTTTATTAATTCGAACAGAATTAGGTAATCCAGGATCTTTAATT GGAGATGATCAAATTTATAACACTATTGTAACAGCTCATGCTTTTATTATAATTTTTTTCATAGTTATACCTATTATAATTGGAGGATTTGGTAATT GATTAGTTCCTTTAATATTAGGAGCTCCTGATATAGCTTTTCCACGAATAAATAATATAAGATTTTGAATACTTCCTCCCTCTTTAATACTTTTAAT TTCAAGAAGAATCGTAGAAAATGGGGCAGGTACTGGTTGAACAGTTTATCCTCCTTTATCTTCTAATATTGCTCATCAAGGTTCATCTGTTGATTTA GCAATTTTTTCTTTACATTTAGCAGGAATTTCATCTATTTTAGGAGCTATTAATTTTATTACTACAATTATTAACATACGAGTAAGAAACTTATCAT TTGATCAAATACCTTTATTTGTATGATCAGTAGGTATTACTGCTTTATTATTACTTTTATCTTTACCTGTATTAGCAGGAGCTATTACTATACTTTT AACTGATCGAAATTTAAATACTTCATTTTTTGACCCTGCTGGAGGAGGAGATCCTATTTTATACCAACATTTATTT

Type material. Holotype: ♂ deposited in the National Museum of Natural History , Smithsonian Institution, Washington, DC, USA ( USNM), illustrated in Fig. 135–136, bears the following four rectangular labels, three white: [ECUADOR-Pich. | Napac- 1000m | 12 Sept. 1976 | leg. S.S.Nicolay], [DNA sample ID: | NVG-19017B02 | c/o Nick V. Grishin], [USNMENT | {QR Code} | 01532380], and one red [HOLOTYPE ♂ | Mnasicles (Remella) | ecua Grishin ].

Type locality. Ecuador: Pichincha Province, Río Napac, elevation 1000 m. Etymology. The name is given for Ecua [dor], the type locality of this species, and is a noun in apposition.

Distribution. Only known from the holotype collected in the Andean region of northern Ecuador.