Petroselinum crispum, SDH (Mill.) SDH

Hýskova, Veronika, Belonozníkov, Katerina, Smeringaiova, Ingrida, Kavan, Daniel, Ingr, Marek & Ryslava, Helena, 2021, How is the activity of shikimate dehydrogenase from the root of Petroselinum crispum (parsley) regulated and which side reactions are catalyzed?, Phytochemistry (112881) 190, pp. 1-12 : 6

3.1. P. crispum root extracts show high SDH activity

One of the reasons why SDH activity is low in plant crude extracts is the presence of phenolics. In general, when exposed to air, plant phenolics are readily oxidized, generating products that form complexes with proteins and inhibit enzyme activity ( Buchanan et al., 2000), as shown in Fig. A.1 . Since the total phenolic content is very high in plants, especially in medicinal herbs ( Tupec et al., 2017), a group of vegetables was chosen to identify a source of high SDH activity from non-photosynthetic tissue. From the group of 8 vegetable sources, the P. crispum root exhibited the highest SDH activity. After 3-step purification procedure specific SDH activity in P. crispum was 470 nmol. min-1. mg -1. This activity was lower than SDH from recombinant sources e.g. ( Tahara et al., 2021) but comparable with SDH from etiolated pea epicotyls ( Balinsky and Davies, 1961), or tomato ( Solanum lycopersicum L.) fruit ( Lourenco and Neves, 1984) or Cucumis sativus L. pulp ( Lourenco et al., 1991).

Figures

Fig. 1. Determination of the type of SDH reaction mechanism based on Lineweaver-Burk diagnostical plots for the direction from SA to DHS (A,B) and from DHS to SA (C,D) Double reciprocal plots are fitted to an equation corresponding to a sequential mechanism.