Ceratomyxa

Laamiri, Sayef, 2017, Myxosporea (Cnidaria: Myxozoa) infecting the saddled seabream Oblada melanura (L. 1758) (Teleostei: Sparidae) and the painted comber Serranus scriba (L. 1758) (Teleostei: Serranidae) in Tunisi, Zootaxa 4269 (1), pp. 61-100: 73-76

publication ID

https://doi.org/10.11646/zootaxa.4269.1.3

publication LSID

lsid:zoobank.org:pub:1266D96E-57FC-4768-A347-A9D395FCDBCF

persistent identifier

http://treatment.plazi.org/id/007687A5-ED17-FFED-AFFC-DE1DFE4BFAB7

treatment provided by

Plazi

scientific name

Ceratomyxa
status

 

Ceratomyxa  sp. 2 ex S. scriba 

Host: Serranus scriba Linnaeus, 1758  painted comber ( Perciformes  : Serranidae  ).

Locality: Mediterranean off Tunisia, Sidi Daoud , Gulf of Tunis (37° 01’ N 10° 55’ E)GoogleMaps  .

Site of infection: Within gall bladder.

Prevalence: The overall prevalence is 6.7% (12/180). The infection rate is distributed as following, 03/2012: 0% (0/30); 04/2012: 0% (0/30); 05/2012: 13.3% (4/30); 06/2012: 13.3% (4/30); 07/2012: 6.7% (2/30); 08/2012: 3.3% (1/30) ( Table 10).

Mean intensity: 71.8±14.5 spores/20µl bile/infected fish (+++++) ( Table 10).

Vouchers: Digitized photos of syntype spores are deposited in the parasitological collection of the Museum National d’Histoire Naturelle (MNHN), Paris, Coll. No. ZS 137. 

Morphological description. Vegetative stages. No early developmental or sporogonic stages are seen for this parasite. The bile is very turbid with cell debris mixed with white grease that bordered at the external wall side of the gall bladder.

Myxospores. Spores typical for the genus Ceratomyxa  (n = 30 fresh spores). Mature spores are reniform, broadly oval to globular in sutural view with anterior margin convex and posterior straight one, cylindrical in apical view ( Figs. 4 View Figure A–B,D–F, 8C–D). They are small in size, measuring 6.2±0.6 (5.2–7.2) µm in length and 12.2±1.4 (10.2–14.0) µm in thickness. Posterior angle is slightly concave to straight 169.8±3.1 (165–173°). In some spores, the posterior margin is nearly parallel to the anterior one with very little valvular taper ( Figs. 4 View Figure B, 8D). The two shell valves are almost equal in size, smoothly ovoid in lateral view with rounded ends. The suture line is straight, visible between valves but not very conspicuous. Sporoplasm, homogenous with sporoplasmosomes, are symmetrically situated and do not fill the entire spore cavity ( Figs. 4 View Figure A–F, 8C–D). It contains two nuclei placed in one side ( Fig. 4 View Figure C). The two polar capsules are in the most cases pyriform, measuring 3.1±0.3 (2.6–3.5) µm in length equaling 49.7% of spore length and 2.5±0.1 (2.3–2.6) µm in width (n = 30), they are placed medially in anterior part of spore in sutural view ( Figs. 4 View Figure A–B, 8C) and centrally of spore cavity in apical view ( Figs. 4 View Figure D–F). The polar filament forms three turns arranged along the longitudinal axis of the capsule.

Taxonomic affinities. Numerous Ceratomyxa  spp. share with the current isolate species one or more morphological similitude especially after the review of genus Leptotheca (Gunter & Adlard 2010)  as we mentioned above. Firstly in the Mediterranean Sea, Ceratomyxa  sp. 2 resembles superficially in shape, form and size to 5 Ceratomyxa  : C. agilis Thélohan, 1892  , C. hepseti Thélohan, 1895  , Ceratomyxa  sp. type 2 ex Epinephelus guaza Siau & Sakiti, 1981  , C. lubati Lubat, Radujkovic, Marques & Bouix, 1989  and Ceratomyxa  sp. 2 ex Sparus aurata Alama-Bermejo, Raga & Holzer, 2011  ( Table 4).

Species Host (s) Locality Spore Polar capsule SL ST PCL PCW

Ceratomyxa  sp. 2 Serranus scriba  Tunisia (Gulf of Tunis) 6.2 12.2 3.1 2.5 

Present stuđy) (5.2/7.2) (10.2/14) (2.6/3.5) (2.3/2.6)

agilis Thélohan (1892)  Dasyatis pastinaca  Međiterranean anđ (6/7) (11/12) ND ND Tyrrhenian coasts

hepseti Thélohan (1895)  Atherina hepsetus Off  France (7/8) (12/15) ND ND

fisheri Jameson (1929)  Hydrolagus colliei Off  USA (5.1/7.1) (9.3/13.3) ND ND

subelegans Lairđ (1953)  *Callogobius atratus, Off New Zealanđ 11.6 22.6 2.5 2.5

Diplocrepis  puniceus (8.6/12.5) (19.6/26.2) (2.1/2.9) (2.1/2.9)

mylionis Ishizaki (1960)  Acanthopagrus schlegelii  Japan 6.2 13.3 2.3 2.3

declivis Meglitsch (1960)  Cyttus novaezelandiae  New Zealanđ (Pacific Ocean) 5.9 14.4 2.4 2

(5.1/6.8) (13.5/15.2) (1.7/2.2) (1.7/2.2)

faba Meglitsch (1960)  Caulopsetta scapha  New Zealanđ (Pacific Ocean) 6.2 12.7 2.4 2.4

(5.6/6.7) (10.7/14.1) (2/3.1) (2/3.1)

pinguis Meglitsch (1960)  *Peltorhamphus New Zealanđ (Pacific Ocean) 9.7 16.4 2.9 2.9

novaezeelandiae (8.3/10.8) (13.7/18.6) (2.4/3.9) (2.4/3.9) Arnoglossus scapha 

coelorhyncha Yoshino & Noble Coelorhinchus  off Irelanđ 6.7(6/8) 11.34 2.01 2.01

1973) coelorhinchus (9/13) (1.5/3) (1.5/3)

Ceratomyxa  sp. type 2 Siau & Sakiti Epinephelus guaza  Međiterranean off Tunisia 5.1 9.3 1.2 1.2 

1981) anđ French

ovalis Kovaljova & Gaevskaya  Trachurus murphyi  Pacific Ocean (6/6.65) (9.97/10.64) (2/2.66) (2/2.66)

1983)

lubati Lubat et al. (1989) Chromis  chromis  off France 6(5.5-7) 14 3 2.75 (12.5/15)

buri, Yokoyama & Fukuđa (2001)  Seriola quinqueradiata  Japan 6.5 (5.5/7.5) 14.3 (11/16.5) 2.4 (2/3) 2.4 (2/3)

cottoidii Reeđ et al. (2007)  Clinus cottoides  South Africa 7.1 (6.5/8) 18.2 (17/22) 2.7 (2.3/3) 2.4 (2/3)

cutmorei Gunter & Ađlarđ (2009)  Epinephelus fasciatus  Australia (Great Barrier Reef) 7 (5/8.5) 16.1 (12/21.5) 2.4 (1.5/3) 2.3 (1.5/3)

ernsti Gunter et al. (2009)  Sillago ciliata  Australia (Great Barrier Reef) 5.76(4.67/6.84) 11.94(9.47/14.79) 1.66(1.3/2.13) 1.58 (1.3/2)

jonesi Gunter et al. (2009)  Pseudolabrus guentheri  Australia (Great Barrier Reef) 5.1(4.1/6.08) 12.99(11.17/16.45) 1.92(1.55/2.3) 1.81(1.42/2.29)

reidi Gunter et al. (2010a)  Chaetodon vagabundus  Australia (Great Barrier Reef) 6.8 (5.8/7.5) 17.5(14.3/20.7) 2.1 (1.7/2.4) 2 (1.7/2.5)

sp 2 Alama-Bermejo et al. (2011) Sparus aurata  Spain 9.9 (8.7/11.4) 20(16.7/24.7) 3.8 (3.2/4.5) 3.8 (3.2/4.5)

Original host.

Although, the dimensions of the polar capsules of C. agilis  have not been given, the available measurements of Ceratomyxa  sp. 2 and this species overlap. However, the spores of C. agilis  are less globular to elongate in shape, its suture line are oblique and its sporoplasm fill the whole spore cavity (specific feature of Leptotheca  spp.). Generally, the average range of spores of C. hepseti  is larger. Furthermore, according to the original description of this species, it was noted that C. hepseti  had a triangular shape in sutural view (no available illustration), so it cannot be identical to the under studied species. The recent species separates from Ceratomyxa  sp. type 2 ex. E. guaza  by having bigger spores and larger pyriform polar capsules. Except the width of the polar capsules, all the measurements between the current species and C. lubati  overlap, but the shape of this later is more arched compared to the globular one of the recent species. Moreover, the sporoplasm of C. lubati  fill the entire spore cavity. C. sp 2 ex S. aurata  can be distinguished from the present species in having larger spores and bigger spherical polar capsules.

Throughout the world, under consideration of the morphological characteristics of the current finding species, 15 representatives of the genus Ceratomyxa  infecting marine fishes are compared to our species. These species are: C. fisheri Jameson, 1929  (formerly L. fisheri  ), C. subelegans Laird, 1953  (formerly L. subelegans  ), C. mylionis Ishizaki, 1960  (syn. L. mylionis  ), C. declivis  C. faba  and C. pinguis  (syn. L. pinguis  ) Meglitsch, 1960, C. coelorhyncha Yoshino & Noble, 1973  (previously L. coelorhyncha  ), C. ovalis Kovaljova & Gaevskaya, 1983  , C. buri Yokoyama & Fukuda, 2001  , C. cottoidii Reed, Basson, Van As & Dyková, 2007  , C. cutmorei Gunter & Adlard, 2009  , C. ernsti  and C. jonesi Gunter, Whipps & Adlard, 2009  , C. reidi Gunter, Burger & Adlard, 2010  and C. hamour Mansour, Al-Qahtani, Al-Quraishy & Abdel-Baki, 2014  ( Tables 4, 6).

Comparative study of morphological and morphometric characteristics of the spore and polar capsule with the mentioned above species confirm that the recent species C. sp. 2 differentiates from all of them. Although, the measurements of polar capsules of C. fisheri  are not given, the range size of body spores for both species overlap. However, according to the original illustration of C. fisheri  , its sporoplasm fill the entire spore cavity and its polar capsules appear to be spherical unlike those of the current species. The spores of C. subelegans  are bigger and possess two spherical polar capsules. The recent finding separates from all species C. mylionis  , C. declivis  , C. faba  , C. pinguis  , C. coelorhyncha  , C. ovalis  , C. lubati  , and C. buri  by having bigger and/or pyriform polar capsules. Furthermore, the posterior margin of C. declivis  is more concave compared to the slightly concave to straight of the present form, C. pinguis  have a bigger spores while those of C. cottoidii  and C. cutmorei  are thicker. Moreover, the polar capsules of the latter are either spherical. C. ernsti  and C. jonesi  differ from the recent species in having almost spherical and smaller polar capsules. The thickness of C. reidi  is larger and its polar capsules are spherical and smaller. The spores of C. hamour  are thicker and less globular in shape than those of our species. Therefore, taking into account all the differences with the closely related species, host and locality new records, the recent myxosporean Ceratomyxa  sp. 2 is designated as a different species, has not previously described from S. scriba  in the Mediterranean Sea.

Kingdom

Animalia

Phylum

Myxozoa

Class

Myxosporea

Order

Bivalvulida

Family

Ceratomyxidae

Loc

Ceratomyxa

Laamiri, Sayef 2017

2017
Loc

cutmorei Gunter & Ađlarđ (2009)

Gunter & Adlard 2009

2009
Loc

cottoidii Reeđ et al. (2007)

Reed et al. 2007

2007
Loc

buri, Yokoyama & Fukuđa (2001)

Yokoyama & Fukuda 2001

2001
Loc

mylionis

Ishizaki 1960

1960
Loc

declivis

Meglitsch 1960

1960
Loc

faba

Meglitsch 1960

1960
Loc

pinguis

Meglitsch 1960

1960
Loc

subelegans Lairđ (1953)

Laird 1953

1953
Loc

fisheri

Jameson 1929

1929
Loc

hepseti Thélohan (1895)

Thelohan 1895

1895
Loc

agilis Thélohan (1892)

Thelohan 1892

1892