taxonID	type	description	language	source
5135879CBF1B2610FF7BF8A658CD31EA.taxon	description	(Figs. 1 – 2) Material examined. Ukraine: Kharkiv Region: Kharkiv, Piatykhatky, 50 ° 05 ' 42 " N 36 ° 15 ' 38 " E, 1. vi. 2019 — pupation outside the mine, Yu. Guglya, ex Cynoglossum officinale (5 puparia); Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 25. vi. 2023 — pupation outside the mine, 29. viii. 2023 — imago, Yu. Guglya, ex Echium vulgaris (1 ♂) (Fig. 2); same locality, 12 and 16. vi. 2023 — pupation outside the mine, Yu. Guglya, ex Cynoglossum officinale (1 ♂ 4 ♀); same locality, 3. vii. 2023 — pupation outside the mine, Yu. Guglya, ex Cynoglossum officinale (1 ♂ 1 ♀); near Kamianka, 49 ° 59 ' N, 37 ° 49 ' E, 3. vi. 2013 — pupation outside the mine, Yu. Guglya, ex Cerinthe minor (2 puparia); near Haidary, 49 ° 37 ' N 36 ° 19 ' E, 14 – 16. x. 2018 — pupation outside the mine, Yu. Guglya, ex Cynoglossum officinale (5 puparia); Donetsk Region: Kryva Luka, 48 ° 52 ' 34 " N, 37 ° 54 ' 37 " E, 3 – 4. vi. 2019 — pupation outside the mine, x. 2019 — dead imago found inside the puparia, Yu. Guglya, ex Cerinthe minor (5 puparia) (Fig. 1). Comments. Females of Agromyza abiens often lay numerous eggs on a single plant (as in fig. 2), resulting in a high degree of plant damage. Four generations were registered in Ukraine, with pupation in June, July, August and October. Imagos were captured from 26 May to 3 September. Host, mine, puparium, cephalopharingeal skeleton, female genitalia discussed in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF1A2610FF7BFDCF5F6236A2.taxon	description	(Figs. 3 – 6, 100 – 106) Material examined. Ukraine: Kharkiv Region: Kharkiv, City Center, Botanical Garden, 50 ° 01 ' N, 36 ° 14 ' E, 11. vi. 2020 — pupation outside the mine, x. 2021 — dead imago found inside the puparia, Yu. Guglya, ex Dipsacus pilosus (1 ♂, 1 ♀ 2 puparia); Donetsk Region: Kryva Luka, 48 ° 52 ' 34 " N, 37 ° 54 ' 37 " E, 04. vi. 2019 — pupation outside the mine, x. 2019 — dead imago found inside the puparia, Yu. Guglya, ex Dipsacus pilosus (1 ♀ 2 puparia). Hosts. Dipsacaceae: Dipsacus fullonum L. (Spencer 1972). Dipsacus pilosus L. — a newly recorded host plant. Mine. (Fig. 3) The solitary larva develops in a greenish-brown blotch mine along the margin of the leaf. Pupation takes place outside the mine in the soil. Puparium. (Figs. 4 – 6) Brown, matt, 3.3 mm, with shallow but distinct segmentation; surface dorsally and ventrally uniformly covered with numerous fine brown spines; several irregular rows of the same spines located along junctures of segments laterally. Posterior spiracles set on dark yellow short stout conical protuberances; yellowish-brown, with 8 minute spherical sessile bulbs in a circular configuration. Ventral portion of last abdominal segment not protruding posteriorly viewed from the side. Anal plate brown and directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 101) Right mouthhook slightly larger dorsally than the left, both with ventro-anterior portion distinctly abducted. Both mouthhooks bear two long sharp accessory teeth uniformly curved ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal cornua and ventral cornu much less so. Indentation index 83. Male genitalia. (Figs. 102 – 104) Only lateral and ventral views of the phallus are depicted in Spencer (1990: figs 913, 914). Other structures are illustrated here. Epandrium is hemispherical. Cercus narrow, elongated with slightly curved anteriorly apical parts. Surstilus elongated with field of numerous prensisetae. Hypandrium is V-shaped with narrow arms. Postgonites massive, with egg-shaped apical half. Ejaculatory apodeme small, with triangular blade. Sperm sac relatively large, drop-shaped, in equal size with blade. Female head. (Fig. 100) Black; fronto-orbital plate visible laterally; 2 ors, 2 ori; lunule of medium height, flattened posteriorly, not reaching the level of the ori a; 1 st fl small. Other features discussed in Spencer (1972) and Papp & Černý (2015). Female genitalia. (Figs. 105, 106) Capsule of spermatheca is relatively of medium size, 0.4 – 0.5 × as high as anterior part of oviscape. Spermathecae not equal in size, brown, oval, without basal collar. Spermathecal duct is very weakly sclerotized. Distribution. Rare European species recorded from Austria, Czech Republic, France, Germany, Great Britain, Slovakia, presumably Hungary (Papp & Černý 2015), Sweeden (Lonsdale 2021). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF17261DFF7BFF1F5B523615.taxon	description	(Figs. 8 – 10, 107 – 113) Material examined. Ukraine: Transcarpathia: 6 km SE Luhy, 760 m a. s. l., 48 ° 01 ' 45 " N, 24 ° 30 ' 59 " E, 12. viii. 2019 — mine with larva collected, Yu. Guglya, ex Trifolium repens (2 puparia); Ivano-Frankivsk Region: Chornohory Massif, 1439 m a. s. l., 48 ° 9 ' N, 24 ° 32 ' E, 9. vi. 2018, Yu. Guglya (1 ♂); Volyn Region: Ostroviye, 51 ° 33 ' 06 " N, 23 ° 47 ' 24 " E, 24. v. 2019, Yu. Guglya (2 ♂); Vinnytsa Region: near Olhopil, 48 ° 07 ' 42 " N, 29 ° 39 ' 25 " E, 11. v. 2019 — pupation outside the mine, Yu. Guglya, ex Trifolium repens (2 ♂ 3 puparia); Poltava Region: near Luchky, 48 ° 57 ' N, 34 ° 9 ' E, 16. v. 2016, Yu. Guglya (1 ♀); Kharkiv Region: Kharkiv, Sokolnyky, 50 ° 01 ' 41 " N, 36 ° 15 ' 35 " E, 17. vi. 2011 — pupation outside the mine, Yu. Guglya, ex Trifolium repens (3 puparia); Donetsk Region: Kryva Luka, 48 ° 52 ' 34 " N, 37 ° 54 ' 37 " E, 2. vi. 2019, Yu. Guglya (1 ♀). Other material listed in Guglya (2011, 2013, 2015, 2017, 2020). Hosts. Fabaceae: Anthyllis L., Medicago L., Melilotus Mill., Onobrychis Mill., Trifolium Tourn ex L., Trigonella L., Vicia L.; Oxalidaceae: Oxalis L. (Benavent-Corai et al. 2005). Mine. (Fig. 8) The solitary larva develops in a white blister-blotch mine in the center of the leaf. Pupation takes place outside the mine in the soil. Puparium. (Figs. 9, 10) Yellowish-brown, matt, 3.0 mm, with deep segmentation; surface uniformly covered with numerous fine brown spines, posterior spiracle set on a conical protuberations in a triangular configuration. Ventral portion of last abdominal segment not protruding posteriorly viewed from the side. Anal plate brown and directed ventrally. Two oval, yellowish-brown appendages developed between the anal plate and posterior spiracles viewed from the side and posteriorly. Cephalopharingeal skeleton. (Fig. 110) Left mouthhook is slightly larger ventrally than the right, both with ventro-anterior portion wide and triangular. Both mouthhooks bear two accessory teeth; both dorsal and right ventral teeth long and strong, uniformly curved ventrally; left medial tooth much shorter, triangular. Parastomal bar is slender, 0.5 × as long as intermediate sclerite. Mouthhooks and intermediate sclerite are strongly sclerotized, parastomal bar, dorsal cornua and ventral cornu much less so. Intermediate sclerite centrally curved, bearing sharp tooth directed posteriorly and located on the middle of ventral side. Indentation index 87. Female head. (Figs. 107, 108) Bicolour; ocellar tubercle, hind margin, 1 st fl, palps are black, the remain dark orange. I vt s and o vt s both in black ground. 1 st fl bearing with orange elongated and dorsally curved hairs. 1 st fl in male larger than in female. Wing. (Fig. 109) Hyaline, with beige undertone and brownish veins; costa ending after R 3 + 4; last section of CuA 1 slightly shorter than penultimate; calypter and margin pale brown, fringe brown. Wing length 2.5 mm. Female genitalia. (Figs. 111 – 113) Capsule of spermatheca medium sized, 0.3 – 0.4 × as high as anterior part of oviscape. Spermathecae unequal in size, brown, oval. Neck of spermatheca is barrel-shaped, 0.3 – 0.4 × as long as height of spermathecae. Spermathecal duct is very weakly sclerotized. Proctiger is wide, 2.4 × as long as maximum width; more sclerotized posteriorly; all setae strong. Distribution. Widespread and abundant European species, reported also from Oriental region (Papp & Černý 2015). Comments. Adults of Agromyza nana were captured in Ukraine from 1 May to 28 August evenly throughout the season.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF17261FFF7BF9D35E083102.taxon	description	(Figs. 13 – 15, 114) Material examined. Ukraine: Kyiv Region: near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 21. vii. 2021 — pupation outside the mine, 1 – 2. viii. 2021 — imago, Yu. Guglya, ex Setaria viridis (4 ♂); Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 1. vii. 2022 — mines with larva collected, 16 – 20. vii. 2022 — imago Yu Guglya, ex Setaria viridis (6 ♂ 2 ♀). Hosts. Poaceae: Setaria viridis (L.) P. Beauv. — the first recorded host plant .. Mine. (Fig. 13) Several larvae form a greenish blotch mine at the leaf apex. Pupation takes place outside the mine in the soil. Puparium. (Figs. 14, 15) Orange-brown, glossy, 2.8 mm long, with deep segmentation; surface quite smooth except for three-row spine bands between all segments, and with two posterior segments wrinkly. Both posterior spiracles are set on one stout conical protuberance; dark brown, with three elongated bulbs set in a circular configuration at acute angles to each other. Anal plate orange-brown, directed ventrally, not protruding above the surface of the puparium when viewed from side. Cephalopharingeal skeleton. (Fig. 114) Right mouthhook is scarcely larger dorsally than the left, both with ventro-anterior portion wide and triangular. Both mouthhooks bear two strong accessory teeth. Mouthhooks and intermediate sclerite strongly sclerotized, dorsal cornua and ventral cornu much less so. Lower dorsal cornu 4 × as wide as upper ones and less sclerotized. The ventral cornu bears an elongated “ closed ” window medially. Indentation index 86. Distribution. Ukraine. Comments. Male and female genitalia depicted and discussed in Guglya (2020).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF15261EFF7BFEE7590930CA.taxon	description	(Figs. 16 – 18, 115 – 127) Material examined. Ukraine: Volyn Region: near Shatsk, 51 ° 28 ' 28 " N, 23 ° 48 ' 27 " E, 23. v. 2019, Yu. Guglya (1 ♀); Kyiv Region: Bila Tserkva, 49 ° 48 ' N, 30 ° 4 ' E, 20. vi. 2015, Yu. Guglya (1 ♂); Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 21. vii. 2021 — mines collected, 22. vii. 2021 — pupation outside the mine, 4. viii. 2021 — imago, Yu. Guglya, ex Setaria viridis (1 ♂ 1 ♀); same locality, 21. vii. 2021, Yu. Guglya (1 ♀); same locality, 29. viii. 2021, Yu. Guglya (1 ♀); Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 1. vii. 2022 — mines with larva collected, 16 – 18. vii. 2022 — imago, Yu. Guglya, ex Setaria viridis (6 ♂ 5 ♀); same locality, 7. vii. 2023 — mines with larva collected, 21 – 25. vii. 2023 — imago, Yu. Guglya, ex Setaria viridis (3 ♂ 1 ♀ 1 larva); Stara Pokrovka, 49 ° 48 ' N, 36 ° 32 ' E, 17. vi. 2023, Yu. Guglya (1 ♂ 1 ♀). Hosts. Poaceae: D. clandestinum (Lam.) Gould, Glyceria canadensis (Michx.) Trin., G. striata (Lam.) Hitchc. (Eiseman and Lonsdale 2018), Dichanthelium scoparium (Lam.) Gould (Eiseman and Lonsdale 2021), Alopecurus pratensis L., Elytrigia repens (L.) Nevski (Grigoryan & Hovhannisyan 2024). Setaria viridis (L.) P. Beauv. — a newly recorded host plant. Mine. (Fig. 16) Several larvae form a dirty whitish blotch apical leaf mine. Pupation takes place outside the mine in the soil. Puparium. (Figs. 17, 18) Orange-brown, glossy, 2.8 mm long, with deep segmentation; surface quite smooth except for three-row spine bands between all segments, and with two posterior segments wrinkly. Both posterior spiracles are set on one stout conical protuberant mounting; dark brown, with three elongated bulbs set in a circular configuration at acute angles to each other. Anal plate brown, scarcely protruding above the surface of the puparium viewed from the side and directed ventrally. Cephalopharingeal skeleton. (Fig. 118) Both mouthhooks equal in size and shape, with sharp abducted ventro-anterior portion directed latero-posteriorly. Both mouthhooks bear two strong accessory teeth; dorsal blunt and ventral sharped. Mouthhooks and most of intermediate sclerite are strongly sclerotized, dorsal cornua and ventral cornu much less so. Lower dorsal cornu 3.5 × as wide as upper ones and less sclerotized. The ventral cornu bears an elongated “ closed ” window posteriorly. Indentation index 80. Male terminalia. (Figs. 122 – 127) See description in Lonsdale (2021). Female head. (Figs. 115, 116) Bicolour; ocellar tubercle, hind margin, palps black, the rest brown; i vt s and o vt s both in brown ground; fronto-orbital plate not visible laterally; 2 ors, 2 ori; 1 st fl black apically, brown basally, rounded bearing short white hairs. Wing. (Fig. 117) Hyaline, with beige veins; costa reaching M 1; last section of CuA 1 0.75 × as long as penultimate; calypter, margin and fringe pale beige. Wing length 2.3 – 2.5 mm. Female genitalia. (Figs. 119 – 121) Proctiger relatively short, equal in length with spermatheca; uniformly cylindrical. Cercus sausage-shaped, 0.56 × as long as proctiger. Several large spines are developed only near posterior margin of proctiger; cercus bears patches of long and medium spines apically and internalaterally. Capsule of spermathecae 0.5 × as high as anterior part of oviscape. Spermathecae equal in size and shape, dark brown, cylindrical, with wrinkled surface; basal half of spermatheca 2 × as wide as apical ones. Neck of spermatheca dark brown, smooth, relatively narrow, 0.3 × as wide as basal part of spermatheca. Internal duct invagination 0.92 × as deep as height of spermatheca. Spermathecal duct weakly sclerotized. Totally seven females of A. parca were reared and three were captured during this investigation. The dissection shows nine females having spermatheca as in fig. 120 a and one as in fig. 120 b. For comparison, dissected female from USA have a shape of spermatheca as in fig 120 b (Owen Lonsdale’s personal communication). Distribution. North America: USA (Lonsdale 2021), Western Asia: Lesser Caucasus (Grigoryan & Hovhannisyan 2024). Central Europe: Ukraine (first record). Comments. In Grigoryan & Hovhannisyan (2024) designated A. parca as “ widespread in the Palaearctic region, China, Japan … ” species, but the author can’t name the source of this information and reference to Nartchuk (2019) was a mistake (Nonna Grigoryan’s personal communication).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF14261EFF7BFEE65F2732EE.taxon	materials_examined	Material examined. Ukraine: Volyn Region: between Pulmo and Pulemets, 51 ° 32 ' 7 " N, 23 ° 45 ' 49 " E, 24. v. 2019, Yu. Guglya (1 ♂ 2 ♀); between Svytiaz and Podmanovo, 51 ° 35 ' 25 " N, 23 ° 45 ' 8 " E, 26. v. 2019, Yu. Guglya (1 ♀); Kyiv Region: NW Makedony, 49 ° 50 ' 32 " N, 31 ° 08 ' 29 " E, 22. v. 2021, Yu. Guglya (7 ♂ 16 ♀); near Balyko-Shchuchynka, 49 ° 56 ' 51 " N, 31 ° 11 ' 01 " E, 23. v. 2021, Yu. Guglya (1 ♀); near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 25. v. 2021, Yu. Guglya (1 ♂); Kharkiv Region: near Rubizhne, 50 ° 07 ' N, 36 ° 46 ' E, 2. v. 2021 — infested stems collected, 5 – 10. v. 2021 — imago, Yu. Guglya, ex Urtica dioica (7 ♂ 5 ♀); near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 10. v. 2021, Yu. Guglya (3 ♂ 2 ♀); same locality, 2 – 3. v. 2020, Yu. Guglya (1 ♂ 6 ♀); Dvorichna, 49 ° 51 ' N, 37 ° 40 ' E, 26. v. 2017, Yu. Guglya (1 ♂ 1 ♀). Comments. Host, mine, puparium, cephalopharingeal skeleton and female genitalia see in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF142619FF7BFCCA5F29342A.taxon	description	(Figs. 11 – 12, 128 – 139) Material examined. Ukraine: Kharkiv Region: near Rubizhne, 50 ° 10 ' N, 36 ° 47 ' E, 2. v. 2021 — stems with puparia collected, v. 2022 — dead imago found, Yu. Guglya, ex Centaurea jacea (1 ♂ 2 ♀); near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 28. iv. 2018 — stems with puparia collected, summer 2022 — dead imago found, Yu. Guglya, ex Centaurea jacea (1 ♂ 1 ♀). Etymology. The name of the new species reflects the fact that the larva leaves as a spem borer. Host. Asteraceae: Centaurea jacea L. Mine. The larva forms bores in the pith of a stem. Pupation takes place within the stem. Puparium. (Figs. 11, 12) Straw-coloured, silky-shining, 3.5 mm long, with scarcely visible segmentation; surface quite smooth, only posterior segment wrinkly. Posterior spiracles set flat on the body cuticle very close but not connected to each other; orange-brown, shining, oval, with median black spine curved dorsally. Eleven to fourteen linear bulbs surround the median spine. Anal plate not protruding above the surface of puparium viewed from the side and directed ventrally. Cephalopharingeal skeleton. (Fig. 131 a – c) Left mouthhook larger dorsally than the right, both with ventro-anterior portion wide and flattened. Right mouthhook bears one strong dorsal accessory tooth, left bears two dorsal teeth 2 × as ventral ones; all teeth uniformly curved ventrally. Both dental sclerites well developed, equal in size, long, narrow, 0.9 × as high as left mouthhook. Mouthhooks, dental sclerites and anterior third of intermediate sclerite are strongly sclerotized, medial and posterior part of intermediate sclerite, dorsal cornua and ventral cornu much less so. Dorsal cornua and ventral cornu wide, with not differentiated “ closed ” windows. Indentation index 60. Adult description. (Figs. 128, 129) Head blackish-brown. Fronto-orbital plate not visible lateraly; 3 ors, 2 ori; orbital setula in two rows, short, proclinate and reclinate together; fronto-orbital plate wide, slightly shining (frontal view); oc tr reaching the level of ors a, slightly shining; frons matt; parafacial narrow; lunule large, reaching the level between ori p and ors a, with distinct furrow; gena and parafacial together medially 0.17 × as wide as maximum height of eye; gena and postgena slightly flattened ventrally; 1 st fl rounded, covered with short thick pale hairs (most visible from side). Upper third of the eye pubescent in male, only few hairs visible along upper eye margin in female. Wing: (Fig. 130) Hyaline, with brown veins; costa reaching M 1; last section of CuA 1 0.6 × as long as penultimate; calypter, margin and fringe dirty white. Wing length 3.0 mm. Mesonotum: Black with yellowish-green undertone, shining viewed from above; scutellum of the same colour, more matt; 0 + 2 dc s, equal in length; acr s in 9 irregular rows at level of anterior dc s; halter blackish-brown; legs blackish-brown, shining. Male genitalia. (Figs. 135 – 139) Distiphallus ovate, with dramatically narrowing anterior half, 0.6 × as wide as its length; with deep, wide invagination between ventro-lateral tubule viewed from below. Mesophallus strongly widening proximally and slightly widening distally, rounded apically viewed from the side. Junction of mesophallus with distiphallus displaced medially. Mesophallus posteriorly located closely to the anterior arm of basiphallus. Basiphallus 0.5 × as long as length of distiphallus, appears trapezoidal because of well sclerotized anterior crossbar; uniformly sclerotized viewed from below; triangular and more sclerotized posteriorly viewed from the side. Phallophorus bulbate viewed from the side. Ejaculatory apodeme elongate, 0.4 × as wide as its length, with strongly asymmetrical blade; 1.15 × as long as length of phallus. Epandrium typical for the genus. Hypandrium stout, sharp anteriorly viewed from below. Metepiphallus trapezoid, with wide rounded invagination viewed from below. Female genitalia. (Figs. 132 – 134) Spermathecae relatively small, 0.15 – 0.18 × as high as height of anterior part of oviscape. Blade of egg-guide relatively wide, 4.7 × as long as maximum width, abducted and rounded apically, with medial membrane bearing about 30 medium and numerous small scales. Outer marginal denticles fine, scarcely visible, directed ventrally. Proctiger generally cylindrical, gradually expanding posteriorly. Cercus narrow, elongated, 0.25 × as long as proctiger; patches of long and medium setae located apically and 2 strong setae internalaterally. Spermathecae unequal in size, brown, narrower than high and flattened basally. Internal duct invagination 0.65 – 0.70 × as deep as height of spermatheca, tapering apically. Distinct lobed basal collar present. Spermathecal duct narrow, very weakly sclerotized. Ventral receptacle pale brown, flat S-shaped, 1.5 × as long as height of spermatheca. Distribution. Ukraine (Kharkiv Region). Comments. Superficially the new species cannot be reliably distinguished from Melanagromyza dettmeri and M. oligophaga. Larvae of all three species feed within stems of Centaurea sp. and puparia are similar except for minute differences in the posterior spiracles. In new species the spiracles are distinctly separated, whereas in M. dettmeri and M. oligophaga they are almost adjoining (Warrington 2024). The male genitalia, in comparison, are distinctly different: basiphallus in M. dettmeri and M. oligophaga is U-shaped because of the absence of a well sclerotized anterior crossbar; the shape of the distiphallus and mesophallus viewed from the side is similar in all three species, but distinctky different when viewed from below. (Papp & Černý 2015: figs 99 D, E; 107 D, E).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF13261BFF7BFB0E5BCF32EE.taxon	description	(Figs. 19 – 21, 140 – 147) Material examined. Ukraine: Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 22. vi. 2023 — stems with larva collected, 24 – 26. vi. 2023 — imago, Yu. Guglya, ex Aquilegia hybr. (2 ♂ 1 ♀); same locality, 29. vi. 2023 — stems with larva collected, 30. vi. 2023 — imago, Yu. Guglya, ex Aquilegia hybr. (1 ♀). Hosts. Ranunculaceae: Aquilegia vulgaris L., Thalictrum flavum L., T. minus L. (Papp & Černý 2015). Mine. (Fig. 19) The larva feeds as an external stem miner in the peduncle. Frass is arranged in large widely spaced grains. Mine tunnels, larva and puparium are well visible from outside. Pupation takes place subepidermally. Puparium. (Figs. 20, 21) Straw-coloured except the dark yellow posterior segment, 3.0 mm long, with readily visible but shallow segmentation; surface quite smooth except for single-row spine bands between all segments. Posterior spiracles set on short cylindrical protuberances that are entirely separated; dark yellow, fan-shaped, with five sessile bulbs arranged in a line. Anal plate scarcely protruding above the surface of puparium when viewed from the side and directed ventro-posteriorly. Spencer (1976) states that the puparium is black, but all reared specimens in my series had straw-coloured puparia. Cephalopharingeal skeleton. (Fig. 143) Left mouthhook distinctly larger dorsally than the right, both with narrow ventro-anterior portion, finger-shaped, abducted ventrally. Both mouthhooks bear two accessory teeth; both dorsal teeth are large, both ventral are small, all sharp, uniformly curved ventrally. Both dental sclerites well developed, equal in size, straight, long, narrow, 0.78 × as high as left mouthhook. Mouthhooks, dental and intermediate sclerites are strongly sclerotized, dorsal cornua and ventral cornu much less so. Intermediate sclerite straight, narrow; bears small right angle tooth on the middle of ventral side. Dorsal and ventral cornua narrow, with undifferentiated “ closed ” windows. Indentation index 73. Female head (Figs. 140, 141) Black, with fronto-orbital plate not visible laterally; 3 ors, 2 ori; 1 st fl of medium size, oval. Gena uniformly rounded, 0.15 × as wide as maximum height of eye. Vibrissa long, scarcely uniformly curved and directed anteriorly, 0.42 × as long as maximum height of eye. Facial carina of medium size, elongated, 0.13 × as wide as minimum width of frons. Wing. (Fig. 142) Hyaline, with brown veins; costa reaching M 1; last section of CuA 1 equal to the penultimate; calypter dark grey, margin and fringe black. Wing length 2.8 mm. Female genitalia. (Fig. 144 – 147) Spermathecae relatively small, 0.14 × as high as anterior part of oviscape. Blade of egg-guide relatively wide, 4 × as long as maximum width, with serrated edge apically and medial membrane with several small scales. Outer marginal denticles fine, scarcely visible, directed posteriorly. Proctiger generally cylindrical, slightly expanding posteriorly. Cercus oval, 0.2 × as long as proctiger; patches of long setae located internalaterally and lateroapically. Spermathecae equal in size, brown, slightly wider than high and flattened basally. Internal duct invagination 0.75 × as deep as height of spermatheca, tapering apically. Distinct basal collar present. Spermathecal duct weakly sclerotized. Ventral receptacle pale brown, S-shaped, 0.5 × as long as egg-guide. Body of receptacle small, spherical, 0.14 × as wide as length of receptacle. Tail of receptacle diverging into two long acute blades. Distribution. British Isles, the Czech Republic, Estonia, Germany, Lithuania and Sweden (Papp & Černý 2015). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF11261AFF7BFCCA5E8631CE.taxon	description	(Figs. 22 – 24, 148 – 154) Material examined. Ukraine: Kharkiv Region: Kharkiv, Sokolnyky, 49 ° 25 ' N, 36 ° 15 ' E, 11. vii. 2020 — stem with larva collected, 15. vii. 2020 — pupation inside the mine, 22. vii. 2020 — imago, Yu. Guglya, ex Lactuca muralis (1 ♂ 1 empty puparium); near Haidary, 49 ° 37 ' N, 36 ° 19 ' E, 25. vii. 2020 — stem with larva collected, 05. viii. 2020 — imago, Yu. Guglya, ex Lapsana communis (1 ♀); Kyiv Region: Balyko-Shchuchynka, 49 ° 56 ' 51 " N, 31 ° 11 ' 01 " E, Yu. Guglya, 23. v. 2021 (1 ♀); Rzhyshchiv, ecostation, 49 ° 57 ' 35 " N, 31 ° 07 ' 17 " E, 24. vii. 2021 — empty puparium inside the stem mine collected, Yu. Guglya, ex Lapsana communis (1 empty puparium). Hosts. Campanulaceae: Campanula L., Jasione L., Phyteuma L ..; Asteraceae: Crepis L., Hieracium L., Hypochoeris L., Lapsana L., Leontodon L., Matricaria L., Mycelis Cass. (syn. Lactuca L.), Prenanthes L., Reichardia Roth, Scorzonera L., Sonchus L., Tragopogon L. (Benavent-Corai et al. 2005). Mine. (Fig. 22) The larva feeds as an external stem miner. Frass is arranged in narrow widely spaced lines. Mine tunnels, larva and puparium are well visible from outside. Pupation takes place subepidermally. Puparium. (Figs. 23, 24) Dark yellow except the black interosegmental bands, 3.0 mm length, with readily visible but shallow segmentation; surface quite smooth except for bands consisting of a single row of large spines and 3 – 4 rows of minute spines between all segments. Posterior spiracles yellowish-black, set on short stout cylindrical protuberances that are entirely separated; dark yellow or brown, fan-shaped, with 11 – 15 sessile bulbs arranged in irregular row. Anal plate brown, with clear contours, protruding above the surface of puparium viewed from the side and directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 151) Right mouthhook larger dorsally and slightly smaller ventrally than the left, both with ventro-anterior portion acute; right abducted anteriorly, left abducted ventrally. Both mouthhooks bear two accessory teeth; both dorsal teeth are large, both ventral are small, all sharp, uniformly curved ventrally. Both dental sclerites well developed, equal in size, strongly curved, long, 1.25 × as high as left mouthhook. Mouthhooks, posterior half of dental sclerites and intermediate sclerite are strongly sclerotized, dorsal cornua and ventral cornua much less so. Intermediate sclerite straight, uniformly widening posteriorly. Indentation index 85. Female head. (Figs. 148, 149) Black, with fronto-orbital plate not visible laterally; 2 ors, 2 ori; 1 st fl small, oval. Gena narrower at rear, 0.15 × as wide as maximum height of eye. Vibrissa of medium length, straight, directed ventro-anteriorly, 0.32 × as long as maximum height of eye. Facial carina of medium size, elongated, with distinct furrow in medial bulb, 0.26 × as wide as minimum width of frons. Wing. (Fig. 150) Hyaline, with greyish undertone, with costa black and the remain veins brown; costa ending after R 3 + 4; last section of CuA 1 1.1 × as long as penultimate; calypter brown, margin and fringe black. Wing length 2.0 mm. Female genitalia. (Fig. 152 – 154) Spermathecae of medium size, 0.24 × as high as anterior part of oviscape. Blade of egg-guide relatively wide, 3.2 × as long as maximum width, acute apically, with medial membrane without any scales. Outer marginal denticles fine, scarcely visible, directed lateroposteriorly. Proctiger generally cylindrical, tapered in the middle. Cercus oval, flattened apically, 0.2 × as long as proctiger; patches of long setae located internalaterally and short setae apically. Spermathecae equal in size, brown, slightly wider than high and flattened basally. Internal duct invagination 0.8 × as deep as height of spermatheca, narrowing medially. Distinct basal collar present. Spermathecal duct weakly sclerotized. Distribution. Austria, British Isles, the Czech Republic, Finland, France, Germany, Hungary, Italy, Latvia, Lithuania, Poland, Slovakia, Sweden, Switzerland (Papp & Černý 2015). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF10261AFF7BFDDF5B0233AA.taxon	description	(Fig. 7) Material examined. Ukraine: Kharkiv Region: Kharkiv, Piatykhatky, 50 ° 05 ' 42 " N 36 ° 15 ' 38 " E, Yu. Guglya, 11. vii. 2021 (1 ♂); near Rubizhne, 50 ° 10 ' N, 36 ° 47 ' E, 18. v. 2019, Yu. Guglya (1 ♀); same location, 19. vii. 2020 — mines with larvae collected, x. 2021 — dried imago found inside the puparium, Yu. Guglya, ex Carlina biebersteini (1 ♂ 1 puparium); Kyiv Region: Rzhyshchiv, 49 ° 58 ' 25 " N, 31 ° 06 ' 20 " E, 22 – 25. vi. 2021 — mines with larvae collected, 27. vi. 2021 — pupation outside the mine, Yu. Guglya, ex Carlina biebersteini (5 puparia). Comments. Hosts, mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF10261AFF7BFC715AA836A6.taxon	materials_examined	Material examined. Ukraine: Transcarpathia: 2.3 km N Kvasy, 905 m a. s. l., deciduous forest, 48 ° 10 ' 13 " N, 24 ° 18 ' 18 " E, 16. viii. 2021 — pupation outside the mine, 2. x. 2021 — imago, Yu. Guglya, ex Stachys sylvatica (1 ♂); E Yasynia, 1040 m a. s. l., 48 ° 12 ' 32 " N, 24 ° 24 ' 56 " E, 24. viii. 2022 — pupation outside the mine, 1. v. 2023 — dried inside the puparium founded, Yu. Guglya, ex Stachys sylvatica (1 ♂); Kyiv Region: near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 23. vi. 2021 — pupation outside the mine, 8. vii. 2021 — imago, Yu. Guglya, ex Ballota nigra (1 ♂); same locality, 28 – 30. viii. 2021 — pupation outside the mine, 19 – 24. ix. 2021, 21. ii. 2022, 9. iii. 2022 — imago, Yu. Guglya, ex Ballota nigra (3 ♂ 2 ♀); same locality, 25. vi. 2021 — pupation outside the mine, 8 – 10. vii. 2021 — imago, Yu. Guglya, ex Lamium album (1 ♂ 2 ♀); same locality, 28. viii. 2021 — pupation outside the mine, 19. ii. 2022, Yu. Guglya, ex ex Leonurus quinquelobatus (1 ♂); same locality, 30. viii. 2021 — pupation outside the mine, 19 and 22. ix. 2021, Yu. Guglya, ex Leonurus quinquelobatus (2 ♂); Sumy Region: Velyka Pysarivka, 50 ° 26 ' N, 35 ° 28 ' E, 23. vi. 2012, Yu. Guglya (2 ♀); Trostianets, 50 ° 28 ' N, 34 ° 55 ' E, 11. vii. 2013, Yu. Guglya (1 ♀); Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 23. vi. 2023 — pupation outside the mine, 7. vii. 2023 — imago, Yu. Guglya, ex Ballota nigra (1 ♂); near Rubizhne, 50 ° 07 ' N, 36 ° 46 ' E, 20. v. 2012, Yu. Guglya (1 ♂). Hosts. Lamiaceae: Leonurus L. (Benavent-Corai et al. 2005), Lamium album L. (Papp & Černý 2016), Betonica officinalis L., Phlomoides tuberosa (L.) Moench, Ballota nigra L. (Guglya 2021). Stachys sylvatica L. — a newly recorded host plant. Comments. Mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF102604FF7BF9755EF83582.taxon	description	(Figs. 25 – 27, 155 – 161) Material examined. Ukraine: Kyiv Region: Rzhyshchiv, the River Dnipro Bank, 49 ° 58 ' 39 " N, 31 ° 06 ' 18 " E, 24. vi. 2021, 26. vi. 2021 — pupation, 8. vii. 2021 — imago, Yu. Guglya, ex Lycopus europaeus (1 ♂, 4 puparia); Kharkiv Region: Pisochyn, the River Merla Bank, 49 ° 57 ' 58 " N, 36 ° 05 ' 13 " E, 29. vi. 2024 — mines with larva collected, 11. vii. 2024 — imago, Yu. Guglya, ex Lycopus europaeus (3 ♀). Hosts. Lamiaceae: Lycopus europaeus L. — the first recorded host plant. Mine. (Fig. 25) The larva forms a brown blotch mine (not apical or marginal as in Phytomyza lycopi Nowakowski!), visibly dried. Pupation takes place either within or outside the mine attached to the leaf surface, but either case on the underside of the leaf. When pupation takes place inside the mine the larva still makes an exit slit. Puparium. (Figs. 26, 27) Orange-brown, glossy, 2.0 mm long, with deep segmentation; surface quite smooth except for narrow brown spine bands and two wrinkled last abdominal segments. Posterior spiracles set on stout conical protuberances that are strongly separated; with three hook-like sessile bulbs set in a circular configuration. Anal plate distinctly protruding above the surface of the puparium viewed from the side; directed ventrally. Cephalopharingeal skeleton. (Fig. 158) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventrally. Both mouthhooks with two accessory teeth; all sharp, uniformly curved and directed ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal cornu very weakly so. Ventral cornu very weakly sclerotized; bears large “ closed ” window medially. Intermediate sclerite straight, uniformly widening posteriorly; bears sharp tooth on the posterior third of ventral margin directed ventrally. Indentation index 80. Female head. (Figs. 155, 156) Black, only frons and gena dark brown; fronto-orbital plate slightly visible laterally; 2 ors, 2 ori; 1 st fl of medium size, round, flattened apically; bears short white pubescence. Gena wider at rear, in widest part 0.32 × as wide as maximum height of eye. Vibrissa short, straight, directed anteriorly, 0.25 × as long as maximum height of eye. Wing. (Fig. 157) Hyaline, with costa black, the remaining veins brown; costa reaching M 1; last section of CuA 1 2.38 × as long as penultimate; calypter grey, margin and fringe black. Wing length 2.0 mm. Female genitalia. (Figs. 159 – 161) Spermathecae of medium size, 0.34 × as wide as height of anterior part of oviscape. Proctiger relatively wide, 2.4 × as long as maximum width; with cylindrical narrowing dramatically on posterior half; anterior half narrow, cylindrical, 0.12 × as wide as posterior half; two pairs of long setae located on posterior margin between cerci. Cercus oval, slightly flattened apically, 0.24 × as long as proctiger; patches of long and short setae located apically, laterally and ventrally. Spermathecae equal in size, brown, wider than high, flattened basally and convex apically. Internal duct invagination wider than high, 0.68 × as deep as height of spermatheca, narrowing medially. Spermathecal duct weakly sclerotized. Distribution. Greece, Hungary (Papp & Černý 2016). Ukraine (first record). Comments. In the end of June 2024 numerous mines with larvae of Amauromyza rameli and Phytomyza lycopi were collected together in Lycopus europaeus thickets. Most of them belonged to P. lycopi and were moustly already empty. In the end of June and in the end of July 2021 mines with larvae and puparia of P. lycopi were collected on Lycopus europaeus in the same location. Mines of A. rameli and P. lycopi can be easily separated from each other based on the position in the leaf. Both species forms visibly dried mine, but in P. lycopi it arranges along leaf margin, and in A. rameli not. (see Figs. 25, 28, 29). Puparia are rather different too. In A. rameli puparium is orange-brown, opaque, posterior spiracles with three hook-like sessile bulbs, and in P. lycopi puparium straw-coloured, transparent with black, two-horned posterior spiracles. (see Figs. 26, 27 and Figs. 213 – 215 in Guglya (2021 )). So, one generation of A. rameli and two generations of P. lycopi were observed in Ukraine to date.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF0E2607FF7BFA675B023222.taxon	description	(Fig. 176, 177) Material examined. Ukraine: Kyiv Region: near Rzhyshchiv, 49 ° 57 ' 39 " N, 31 ° 07 ' 47 " E, 22 – 23. vi. 2021 — pupation outside the mine, 5 – 7. vii. 2021 — imago, Yu. Guglya, ex Cerastium glomeratum (2 ♂ 6 ♀); near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 23 – 25. vi. 2021 — pupation outside the mine, 6 – 8. vii. 2021 — imago, Yu. Guglya, ex Silene latifolia spp. alba (3 ♂ 1 ♀); same locality, 1. ix. 2021 — pupation outside the mine, 19. ix. 2021 — imago, Yu. Guglya, ex Silene latifolia spp. alba (2 ♂); Rzhyshchiv, deciduous forest, 49 ° 58 ' 25 " N, 31 ° 06 ' 20 " E, 25 – 28. vi. 2021 — pupation outside the mine, 7 – 9. vii. 2021 — imago, Yu. Guglya, ex Stellaria aquatica (2 ♂ 2 ♀); Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 15. vi. 2021 — pupation outside the mine, 26 – 27. vi. 2021 — imago, Yu. Guglya, ex Gypsophila elegans (2 ♀); near Rubizhne, 50 ° 07 ' N, 36 ° 46 ' E, 21. v. 2017, Yu. Guglya (1 ♀); near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 28. vi. 2021 — mine with larva collected, x. 2021 — dried imago found inside the puparium, Yu. Guglya, ex Silene latifolia spp. alba (2 ♀); same locality, 10. vi. 2020 — pupation outside the mine, 22. vi. 2020 — imago, Yu. Guglya, ex Silene baccifera (1 ♀); Donetsk Region: Kryva Luka, 48 ° 52 ' 34 " N, 37 ° 54 ' 37 " E, 4. vi. 2019 — pupation outside the mine, 14. vi. 2019 — imago, Yu. Guglya, ex Phlox drummondii (1 ♀ 4 puparia). Hosts. Caryophyllaceae: Agrostemma L., Cerastium Toum ex L., Dianthus L., Gypsophila L., Moehringia L., Saponaria L., Silene L., Stellaria L.; Amaranthaceae: Beta L., Spinacia L. (Benavent-Corai et al. 2005); Vaccaria Wolf. (Dempewolf 2004); Amaranthaceae: Beta vulgaris L., Spinacia oleracea L. (Scheffer 1999); Brassicaceae: Lepidium draba L.; Asteraceae: Catananche caerulea L. (Gil-Ortiz et al. 2009), Bidens L. (Lonsdale et al. 2023); Polymoniaceae: Phlox drummondii Hook. — a newly recorded host plant. Female genitalia. (Figs. 176, 177) Spermathecae of medium size, 0.23 × as high as height of anterior part of oviscape. Proctiger relatively wide, 2.4 × as long as maximum width; cylindrical in posterior half, anterior half conical; pair of long setae located on posterior margin between cerci. Cercus as long as wide, with shallowly rounded apex, 0.3 × as long as proctiger; patches of short setae located apically, long setae located apically and internolaterally. Comments. Hosts, mine, puparium, cephalopharingeal skeleton and spermathecae depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF0D2607FF7BFD075EB437E5.taxon	description	(Figs. 30 – 32, 162 – 168) Material examined. Ukraine: Transcarpathia: Nova Stuzhytsa, 49 ° 02 ' 31 " N, 22 ° 34 ' 53 " E, 07 – 08. vii. 2019 — pupation outside the mine, dried imago found inside the puparium, Yu. Guglya, ex Campanula sp. (1 ♀); Ivano-Frankivsk Region: near Zarosliak, Polonyna Pozhyzhevska, S slope, 48 ° 09 ’ N, 24 ° 31 ’ E, 12. vi. 2018, Yu. Guglya (1 ♀); Kyiv Region: Rzhyshchiv, 49 ° 58 ' 02 " N, 31 ° 06 ' 38 " E, 21. vii. 2021, Yu. Guglya (1 ♂); Rzhyshchiv, deciduous forest boarder, 49 ° 58 ' 25 " N, 31 ° 06 ' 20 " E, 25. vi. 2021 — pupation outside the mine, 6 and 11. vii. 2021 — imago, Yu. Guglya, ex Campanula rapunculoides (2 ♂ 1 puparium); same locality, 25 – 26. vi. 2021 — pupation outside the mine, 7. vii. 2021 — imago, Yu. Guglya, ex Campanula trachelium (1 ♂ 2 ♀); same locality, 23. vii. 2021 — pupation outside the mine, 4 and 20. viii. 2021 — imago, Yu. Guglya, ex Campanula trachelium (2 ♂ 3 puparia); Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 10 – 11. vi. 2024 — pupation outside the mine, 21. vi. 2024 — imago, Yu. Guglya, ex Campanula persicifolia (4 ♂); N Kharkiv, Piatykhatky, 50 ° 05 ' 45 " N, 36 ° 15 ' 32 " E, 11. vii. 2021 — pupation outside the mine, 22. vii. 2021 — imago, Yu. Guglya, ex Campanula trachelium (2 ♀). Hosts. Campanulaceae: Adenophora Fisch., Campanula L., Legousia Durande, Phyteuma L. (Benavent-Corai et al. 2005). Mine. (Fig. 30) The solitary larva forms a greenish-white upper surface blotch mine, which eventually becomes brown. Pupation takes place outside the mine. Puparium. (Figs. 31, 32) Bright yellow, glossy, 2.0 mm long, with deep segmentation; surface quite smooth. Posterior spiracles brown, set on yellow conical protuberances that are strongly separated; with four hook-like sessile bulbs set in a circular configuration. Anal plate not protruding above the surface of the puparium viewed from the side; directed ventrally. Two sharp conical ventral tubercles developed on each side of the anal plate viewed from the side and posteriorly. Cephalopharingeal skeleton. (Fig. 165) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute and abducted ventrally. Right mouthhook bears two accessory teeth, left bears one; all teeth are large, sharp, uniformly curved ventrally. Mouthhooks strongly sclerotized, intermediate sclerite and dorsal and ventral cornua much less so. Intermediate sclerite straight and wide. Dorsal cornu gradually curved and narrowed. Ventral cornu very weakly sclerotized; bearing a “ closed ” window posteriorly. Indentation index 84. Female head. (Figs. 162, 163) Black, with fronto-orbital plate slightly visible laterally; 3 ors, 2 ori; 1 st fl small, oval. Gena wider at rear, at widest part 0.24 × as wide as maximum height of eye. Wing. (Fig. 164) Greyish; with dark grey veins. Costal veins ending afrer M 1; last section of CuA 1 3.5 × as long as penultimate; first cross-vein at midpoint of discal cell; calypter and fringe grey, margin black. Wing length 1.6. mm. Female genitalia. (Figs. 166 – 168) Spermathecae of medium size, 0.35 × as wide as height of anterior part of oviscape. Proctiger short, generally cylindrical, tapered in the middle. Several long setae located separately along posterior margin. Cercus oval, flattened apically and basally, 0.55 × as long as proctiger; patches of long setae located laterally and brush of strong short setae located apically. Spermathecae equal in size, brown, wider than high and flattened basally. Internal duct invagination wide, oval 0.73 × as deep as height of spermatheca. Spermathecal duct is weakly sclerotized but distinctly wrinkled. Distribution. Widely distributed European species (Papp & Černý 2016). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF0C2606FF7BFF1F59353759.taxon	description	(Figs. 33 – 36, 169 – 175) Material examined. Ukraine: Transcarpathia: Nova Stuzhytsa, 49 ° 2 ' 8 " N, 22 ° 34 ' 39 " E, 9 – 10. vii. 2019 — pupation outside the mine, 24. viii. 2019 — imago, Yu. Guglya, ex Verbascum nigrum (2 ♂ 2 ♀ 3 puparia); Kyiv Region: Kyiv, Irpin, 50 ° 30 ' 42 " N, 30 ° 15 ' 40 " E, 21. vii. 2019 — pupation outside the mine, Yu. Guglya, ex Verbascum nigrum (1 ♂ 2 puparia); Kyiv, Lysa Hora location, 50 ° 23 ' 42 " N, 30 ° 32 ' 53 " E, vi. 2019 — pupation outside the mine, A. Gumovski, ex Scrophularia nodosa (1 puparium); S Rzhyshchiv, 49 ° 56 ' 07 " N, 31 ° 02 ' 50 " E, 26. vi. 2021 — pupation outside the mine, 2. ix. 2021 — imago, Yu. Guglya, ex Scrophularia nodosa (1 ♂); Kharkiv Region: near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 9. vi. 2020 — pupation outside the mine, 29. vi. 2020 — imago, Yu. Guglya, ex Verbascum densiflorum (1 ♀); same locality, 18. vi. 2019 — pupation outside the mine, 8. viii. 2019 — imago, Yu. Guglya, ex Verbascum densiflorum (1 ♀); same locality, 18. vi. 2019 — pupation outside the mine, 20 – 23. viii. 2019 — imago, Yu. Guglya, ex Verbascum densiflorum (3 ♀ 1 puparium); same locality, 29. vi. 2020 — pupation outside the mine, 3. ix. 2020 — imago, Yu. Guglya, ex Verbascum densiflorum (1 ♂ 1 puparium); same locality, 10. vi. 2020 — pupation outside the mine, 21. ix. 2020 — imago, Yu. Guglya, ex Verbascum densiflorum (1 ♀); same locality, 2. vii. 2012 — pupation outside the mine, Yu. Guglya, ex Verbascum densiflorum (4 puparia); Stara Pokrovka, 49 ° 48 ' N, 36 ° 32 ' E, 20. vi. 2012 — pupation outside the mine, Yu. Guglya, ex Verbascum densiflorum (1 puparium); near Rubizhne, 50 ° 06 ' N, 36 ° 46 ' E, 19. vii. 2020 — dead larvae found inside the mine, Yu. Guglya, ex Scrophularia nodosa (3 larvae). Hosts. Scrophulariaceae: Buddleja Houst. ex L., Scrophularia Turn. ex L., Verbascum L. (Benavent-Corai et al. 2005). Mine. The solitary larva forms a white linear-blotch mine (Fig. 33), which the initial linear portion is sometimes obscured by the later blotch mine (Fig. 34); on the upper leaf surface. Pupation takes place outside the mine in a soil. Puparium. (Figs. 35, 36) Dark orange, glossy, 3.2 – 3.5 mm long, with deep segmentation; surface quite smooth, glossy. Posterior spiracles brown, set on conical strongly separated protuberances; with four hook-like sessile bulbs set in a circular configuration. Anal plate protruding above the surface of the puparium viewed from the side; directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 172) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute and abducted ventrally. Both mouthhooks bear two accessory teeth; all are long, sharp, uniformly curved ventrally. Parastomal bar narrow, long, 0.65 × as long as length of intermediate sclerite. Mouthhooks and anterior ventral half of intermediate sclerite are strongly sclerotized, the remain of intermediate sclerite, parastomal bar and dorsal and ventral cornua much soles sclerotized. Intermediate sclerite straight, dramatically widening in posterior third. Indentation index 90. Female head. (Figs. 169, 170) Orange, with post gena brown, o vt s on brown ground, and i vt s between orange and brown; fronto-orbital plate visible laterally; 3 ors, 3 ori; 1 st fl small, round, covered by thick yellow pubescence directed anteriorly. Gena wider at rear, in widest part 0.3 × as wide as maximum height of eye. Vibrissa short, straight, directed ventro-anteriorly, 0.27 × as long as maximum height of eye. Wing. (Fig. 171) Hyaline, with yellow undertone, with beige veins; costa reaching M 1; last section of CuA 1 2.6 × as long as penultimate; calypter and margin yellow, fringe black. Wing length 3.5 mm. Female genitalia. (Figs. 173 – 175) Spermathecae of medium size, 0.22 × as high as anterior part of oviscape. Proctiger narrow and long, generally cylindrical, gradually widening posteriorly. Several long and medium setae located along posterior margin separately. Cercus oval, 0.15 × as long as length of proctiger; patches of long and medium setae located internalaterally and apically. Spermathecae equal in size, brown, highly asymmetrical, drop-shaped, slightly wider than high and flattened basally. Internal duct invagination 0.45 × as deep as height of spermatheca, shifted to the narrow part of spermatheca. Spermathecal duct dramatically widening near capsule of spermatheca; weakly sclerotized but distinctly wrinkled. Distribution. European species which is reported from Great Britain to Romania and Moldova and from Spain and Italy to Sweden (Papp & Černý 2016). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF0A2600FF7BFF1F5AA83206.taxon	description	(Fig. 37) Material examined. Ukraine: Kyiv Region: near Rzhyschiv, 49 ° 57 ' 05 " N, 31 ° 05 ' 26 " E, 20. vii. 2021 — mines with puparia collected, 30. vii. 2021 — imago, Yu. Guglya, ex Zea mays (1 ♀); Kharkiv Region: Pisochyn, 49 ° 57 ' 58 " N, 36 ° 5 ' 13 " E, 29. vi. 2024 — mine with puparium collected, 03. vii. 2024 — imago, Yu. Guglya, ex Lolium arundinaceum (1 ♂). Mine. (Fig. 37) Several larvae form a large brown blotch mine, which appears dry. Pupation takes place inside the mine. Comments. Puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF0A2600FF7BFDE35AA833F2.taxon	materials_examined	Material examined. Ukraine: Transcarpathia: E Yasynia, valley of Ozerne Lake, 1300 m a. s. l., 49 ° 11 ' 22 " N, 24 ° 25 ' 47 " E, 20. viii. 2022 — mine with puparum collected, 25. viii. 2022 — imago, Yu. Guglya, ex Gentiana asclepiadea (1 ♂); foothills of Dantsyzh Mt., 1600 m a s. l., 48 ° 07 ' 40 " N, 24 ° 31 ' 6 " E, 22. viii. 2022 — mines with puparia collected, 31. viii & 4. ix. 2022 — imago, Yu. Guglya, ex Gentiana lutea (2 ♂ 1 ♀); Ivano-Frankivsk Region: near Zarosliak, 1400 m a. s. l., 48 ° 10 ' 10 " N, 24 ° 31 ' 24 " E, 17. viii. 2021 — mines with puparia collected, 31. viii. 2021 — imago, Yu. Guglya, ex Gentiana asclepiadea (2 ♀ 1 puparium); foothills of Dantsyzh Mt., 1460 m a s. l., 48 ° 08 ' N, 24 ° 32 ' E, 26. viii. 2022 — mines with puparia collected, 1 - 4. ix. 2022 — imago, Yu. Guglya, ex Gentiana asclepiadea (4 ♂ 6 ♀). Comments. Mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF0A2603FF7BFBB75AA836A2.taxon	materials_examined	Material examined. Belgium: Edegem, 51 ° 09 ' N, 04 ° 27 ' E, 20. v. 2018 — mines with puparia collected, 25. v. 2018 — imago, I. Moskalets, ex Cirsium vulgare (2 ♂); Ukraine: Transcarpathia: Rakhiv, 430 m a. s. l., 48 ° 03 ' N, 24 ° 13 " E, 8. v. 2018 — mines with puparia collected, 15. v. 2018 — imago, Yu. Gugllya, ex Taraxacum officinale (3 ♂ 1 ♀); 1 km N Rakhiv Center, 640 m a. s. l., 48 ° 03 " N, 24 ° 13 " E, 9. v. 2018 — mines with puparia collected, Yu. Guglya, ex Centaurea sp. (2 puparia); Rakhiv district, near Luh, 47 ° 56 ' 20 " N, 24 ° 06 ' 40 " E, 10. v. 2018 — mine with puparium collected, Yu. Guglya, ex Petasites hybridus (1 puparium); Nova Stuzhytsa, 440 m a. s. l., 49 ° 02 ' 08 " N, 22 ° 34 ' 39 " E, 4. vii. 2019 — mine with puparium collected, Yu. Guglya, ex Centaurea jacea (1 puparium); near Lubnya, 530 m a. s. l., 49 ° 00 ' 74 " N, 22 ° 43 ' 75 " E, 5. vii. 2019 — mine with puparium collected, Yu. Guglya, ex Eupatorium cannabinum (1 puparium); near Kamianytsa, Antalovska Polyana, 934 m a. s. l., 48 ° 40 ' N, 22 ° 29 ' E, 22. viii. 2017 — mines with empty puparium collected, Yu. Guglya, ex Sonchus sp. (1 empty puparium); near Luhy, 760 m a. s. l., 48 ° 01 ' 45 " N, 24 ° 30 ' 59 " E, 12. viii. 2019 — mines with puparia collected, Yu. Guglya, ex Petasites hybridus (1 puparium); Ivano-Frankivsk Region: foothills of Dantsyzh Mt., 1600 m a s. l., 48 ° 08 ' N, 24 ° 32 ' E, 26. viii. 2022 — mines with puparia collected, Yu. Guglya, ex Jacobaea subalpina (3 puparia); Volyn Region: near Shatsk, 51 ° 28 ' 28 " N, 23 ° 48 ' 27 " E, 23. v. 2019 — mine with puparium collected, Yu. Guglya, ex Centaurea sp. (1 puparium); same locality, 23. v. 2019 — mine with puparium collected, 1. vi. 2019 — imago, Yu. Guglya, ex Cirsium arvense (1 ♀); between Zhabky and Dachne, 50 ° 49 ' 26 " N, 25 ° 22 ' 31 " E, 8. vi. 2019 — mines with puparia collected, Yu. Guglya, ex Rorippa austriaca (1 puparium); Vinnytsa Region: Chechelnyk, 48 ° 12 ' 53 " N, 29 ° 24 ' 37 " E, 29. vi. 2019 — mine with puparium collected, Yu. Guglya, ex Lactuca serriola (1 puparium); Kyiv Region: Kyiv, near Pecherska metro staintion, 50 ° 25 ' 39 " N, 30 ° 32 ' 20 " E, 22. vii. 2019 — mine with puparium collected, Yu. Guglya, ex Scorzoneroides autumnalis (1 ♂); Kyiv, Trukhaniv Island, 50 ° 27 ' 48 " N, 30 ° 32 ' 42 " E, 24. vii. 2019 — mines with puparia collected, Yu. Guglya, ex Cirsium vulgare (1 ♂); same locality and date, 31. vii & 1. viii. 2019 — imago, Yu. Guglya, ex Taraxacum officinale (1 ♂ 1 ♀); near Tarasivka, 50 ° 09 ' 49 " N, 30 ° 35 ' 49 " E, 22. v. 2019 — mines with puparia collected, 28. v. 2019 — imago, Yu. Guglya, ex Taraxacum officinale (2 ♂ 1 ♀); Mironivka District, Tulintsy, 49 ° 49 ' N, 31 ° 10 ' E, 23. vi. 2021 — mine with puparium collected, Yu. Guglya, ex Papaver somniferum (1 ♂); Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 2. v. 2022 — mines with puparia collected, 11. v. 2022 — imago, Yu. Guglya, ex Cynoglossum officinale (5 ♂ 1 ♀); same locality, 27. v. 2019 — mine with puparium collected, 29. v. 2019 — imago, Yu. Guglya, ex Centaurea sp. (1 ♀); same locality, 30. v. 2019 — mine with puparium collected, 2. vi. 2019 — imago, Yu. Guglya, ex Cirsium arvense (1 ♂); same locality, 30. v. 2019 — mine with puparium collected, 5. vi. 2019 — imago, Yu. Guglya, ex Taraxacum officinale (1 ♂); same locality, 3. vi. 2020 - mine with puparium collected, 5. vi. 2020 — imago, Yu. Guglya, ex Scorzoneroides autumnalis (1 ♀); same locality, 10. vi. 2020 — mine with puparium collected, 14. vi. 2020 — imago, Yu. Guglya, ex Echium vulgare (1 ♂); same locality, 17. vi. 2023 — mine with puparium collected, Yu. Guglya, ex Tropaeolum majus (1 empty puparium); same locality, 18. vi. 2021 — mines with puparia collected, 22. vi. 2021 — imago, Yu. Guglya, ex Petunia × hybrida (3 ♂ 1 ♀); same locality, 19. vi. 2022 — mines with puparia collected, 20 & 21. vi. 2022 — imago, Yu. Guglya, ex Gazania rigens (2 ♀); same locality, 20. vi. 2023 — mines with larvae collected, 23. vi. 2023 — pupation inside the mine, 28 – 29. vi. 2023 — imago, Yu. Guglya, ex Trifolium repens (2 ♂ 2 ♀ 3 puparia); same locality, 25. vi. 2022 — mine with puparium collected, 30. vi. 2022 — imago, Yu. Guglya, ex Papaver rhoeas (1 ♀); same locality, 30. vi. 2023 — mines with puparia collected, 9 & 15. vii. 2023 — imago, Yu. Guglya, ex Verbena hybrida (1 ♂ 1 ♀); same locality, 20. vii. 2011 — mines with puparia collected, 21 – 22. vii. 2011 — imago, Yu. Guglya, ex Psephelus dealbatus (1 ♂ 1 ♀); same locality, 13. vi. 2024 — mines with puparia collected, 15. vi. 2024 — imago, Yu. Guglya, ex Lobularia maritima (2 ♀); same locality, 30. v. 2024 — mine with larva collected, 15. vi. 2024 — empty puparium found, Yu. Guglya, ex Tanacetum vulgare (1 puparium); Kharkiv, Velyka Danylivka, 50 ° 2 ' 43 " N, 36 ° 19 ' 54 " E, 29. v. 2016 — mines with puparia collected, 6. vi. 2016 — imago, I. Moskalets, ex Sonchus sp. (10 ♂ 2 ♀); same locality, 1. vi. 2016 — mines with puparia collected, 7. vi. 2016 — imago, I. Moskalets, ex Pisum sativum (14 ♂ 5 ♀); same locality, 15. vi. 2013 — mine with puparium collected, 19. vi. 2013 — imago, I. Moskalets, ex Senecio vulgaris (1 ♂); same locality, 27. vi. 2023 — mines with puparia collected, 30. vi & 2 – 3. vii. 2023 — imago, I. Moskalets, ex Lavandula angustifolia (2 ♂ 3 ♀); same locality, 27. vi. 2023 — mines with puparia collected, 6 & 7. vii. 2023 — imago, I. Moskalets, ex Allium cepa (1 ♂ 1 ♀); same locality, 25. vii. 2015 — mine with puparium collected, 3. viii. 2015 — imago, I. Moskalets, ex Sonchus asper (1 ♀); same locality, 30. vii. 2019 — mine with puparium collected, 2. viii. 2019 — imago, I. Moskalets, ex Mentha sp. (1 ♂); same locality, 6. viii. 2015 — mines with puparia collected, 8. viii. 2015 — imago, I. Moskalets, ex Sonchus asper (2 ♀); N Kharkiv, Piatykhatky, 50 ° 05 ' 42 " N 36 ° 15 ' 38 " E, 29. v. 2019 — mine with puparium collected, 30. v. 2019 — imago, Yu. Guglya, ex Echium vulgare (1 ♀); same locality, 29. v. 2019 — mines with puparia collected, 30. v. 2019 — imago, Yu. Guglya, ex Taraxacum officinale (1 ♂ 3 ♀); same locality, 29. v. 2019 — mine with puparium collected, 31. v. 2019 — imago, Yu. Guglya, ex Cyclahaena xanthiifolia (1 ♂); same locality, 29. v. 2019 — mine with puparium collected, 2. vi. 2019 — imago, Yu. Guglya, ex Rudbeckia hybr. (1 ♀); same locality, 29. v. 2019 — mine with puparium collected, 5. vi. 2019 — imago, Yu. Guglya, ex Arctium tomentosum (1 ♂); same locality, dates and collector, ex Lactuca tatarica (1 ♂); same locality, 4. vi. 2020 — mine with puparium collected, 12. vi. 2020 — imago, Yu. Guglya, ex Asperugo procumbens (1 ♀); near Rubizhne, 50 ° 07 ' N 36 ° 46 ' E, 29. v. 2010 — mines with puparia collected, 5 & 8. vi. 2010 — imago, Yu. Guglya, ex Lactuca serriola (2 ♂ 1 ♀); same locality, 29. v. 2010 — mines with puparia collected, 6 & 11. vi. 2010 — imago, Yu. Guglya, ex Sonchus oleraceus (2 ♀); near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 16. vi. 2019 — mines with puparia collected, Yu. Guglya, ex Tanacetum vulgare (4 puparia); same locality, date and collector, ex Rorippa sp. (4 puparia); Donetsk Region: Kryva Luka, 48 ° 52 ' 34 " N 37 ° 54 ' 37 " E, 1 – 3. vi. 2019 — mines with puparia collected, Yu. Guglya, ex Lepidium draba, Cicorium intybus, Centaurea jacea, Senecio erucifolius & Lactuca serriola (5 puparia); same locality, 1. vi. 2019 — mine with puparium collected, 8. vi. 2019 — imago, Yu. Guglya, ex Erysimum cheiranthoides (1 ♂); same locality, 2 – 3. vi. 2019 — pupation inside the mine, 17. vi. 2019 — imago, Yu. Guglya, ex Tanacetum sp. (1 ♀); Crimea: Foros, 44 ° 23 ' N, 33 ° 47 ' E, 27. iv. 2014 — imago, I. Moskalets, ex Sonchus oleraceus (1 ♀). Comments. Mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF092602FF7BF88659C334BE.taxon	description	(Figs. 38 – 40, 181 – 187) Material examined. Ukraine: Transcarpathia: 5 km N Kvasy, 1200 m a. s. l., 48 ° 09 ' 21 " N, 24 ° 20 ' 19 " E, 13. viii. 2021 — mines with puparia collected, 23. viii. 2021 — imago, Yu. Guglya, ex Primula sp. (1 ♀); same locality, 18 & 19. viii. 2019 — numerous mines with puparia collected, Yu. Guglya, ex Primula sp. (6 puparia); Ivano-Frankivsk Region: near Zarosliak, 1400 m a. s. l., 48 ° 10 ' 10 " N, 24 ° 31 ' 24 " E, 17. viii. 2021 — mines with puparia collected, Yu. Guglya, ex Primula sp. (2 puparia). Hosts. Primulaceae: Primula bulleyana Forrest, P. elatior (L.) Hill, P. farinosa L., P. veris L., P. vulgaris Huds. (Papp & Černý 2019). Mine. (Fig. 38) The larva forms a white upper surface linear mine with widely separated grains of frass. When several larva feed in one leaf, mines can unite and appear as a single blotch mine. Pupation takes place within the mine on the lower leaf surface, with anterior spiracles stretching through the leaf cuticle. Puparium. (Figs. 39, 40) Orange with anterior and posterior spiracles, are black, glossy; 2.5 mm long, with shallow but distinct segmentation; surface smooth except for the wide wrinkled spine bands. Posterior spiracles two-horned, divergent latero-posteriorly, set on stout high separated conical protuberances. Anal plate distinctly protruding above the surface of the puparium viewed from the side; directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 184) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute and abducted ventrally. Both mouthhooks bear two wide accessory teeth; all sharp, uniformly curved ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal and ventral cornua much less so. Intermediate sclerite straight, slightly widening posteriorly, with finger-shaped appendage located ventro-posteriorly. Dorsal and ventral cornua equal in width. Ventral cornu bears “ closed ” window medially. Indentation index 82. Female head. (Figs. 181, 182) Black, except pale brown gena and fronto-orbital plate and yellow labellum, with fronto-orbital plate visible laterally; 2 ors, 1 ori; 1 st fl small, oval, covered with bright pubescence directed dorsally. Gena wider at rear, 0.45 × as wide as maximum height of eye. Vibrissa and gn s equal in length, long and strong, 0.54 × as long as maximum height of eye. Wing. (Fig. 183) Hyaline, with greyish undertone; veins brownish; costa reaching R 3 + 4; second cross vein absent; calypter, margin and fringe beige. Wing length 2.0 mm. Female genitalia. (Figs. 185 – 187) Proctiger cylindrical. Pair of long setae located on posterior margin between cerci. Cercus oval, wider apically and narrower basally, 0.3 × as long as length of proctiger; medium and short setae located apically. Ventral receptacle S-shaped, with flattened capsule, short, straight narrow basal connecting tube and massive tail. Distribution. Bulgaria (Beiger 1980), Austria, Belgium, the Czech Republic, Denmark, Finland, France, Germany, Great Britain, Ireland, Italy, Lithuania, Montenegro, The Netherlands, Norway, Poland, Spain incl. Canary Islands, Sweden, Switzerland (Papp & Černý 2019). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF082602FF7BFB7B5B023632.taxon	materials_examined	Material examined. Ukraine: Kyiv Region: S Rzhyshchiv, 49 ° 56 ' 01 " N, 31 ° 02 ' 36 " E, 27. vi. 2021 — pupation outside the mine, 6. vii. 2021 — imago, Yu. Guglya, ex Hyoscyamus niger (1 ♀); Kharkiv Region: Kharkiv: City Center, 50 ° 00 ' N, 36 ° 14 ' E, 10. ix. 2022 — pupation outside the mine, 3. ii. 2023 — imago, Yu. Guglya, ex Ajuga repens (1 ♂ 2 ♀); Velyka Danylivka, 10. vii. 2021 — pupation outside the mine, 18. vii. 2021 — imago, M. Fandikova, ex Tropaeolum majus (4 ♂ 3 ♀). Comments. Hosts, mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF08260DFF7BF9F75AB1342A.taxon	description	(Figs. 41 – 43, 188 – 195) Material examined. Ukraine: Kharkiv Region: N Kharkiv, Piatykhatky, 50 ° 05 ' 42 " N, 36 ° 15 ' 38 " E, 7 – 8. vi. 2020 — pupation outside the mine, 18 – 20. vi. 2020 — imago, Yu. Guglya, ex Artemisia vulgaris (2 ♂ 2 ♀); same locality, 21 – 23. vi. 2021 — pupation outside the mine, 2 – 6. vii. 2021 — imago, Yu. Guglya, ex Artemisia vulgaris (3 ♂ 5 ♀). Hosts. Asteraceae: Artemisia L. (Benavent-Corai et al. 2005). Mine. (Fig. 41) The solitary larva forms a yellowish-green upper surface linear leaf mine with long strips of frass arranged along both mine margins. Pupation takes place outside the mine. Puparium. (Figs. 42, 43) Yellow, glossy, 2.0 mm long, with distinct segmentation; surface quite smooth. Posterior spiracles brown, set on yellow, stout, separated conical protuberances; with four hook-like sessile bulbs set in a circular configuration. Anal plate scarcely protruding above the surface of the puparium viewed from the side; directed ventrally. Two shallow round appendages developed between the anal plate and posterior spiracles viewed from the side and posteriorly. Cephalopharingeal skeleton. (Fig. 192) Right mouthhook larger dorsally than the left, both with ventro-anterior portion flattened and abducted anteriorly. Both mouthhooks bear two large accessory teeth; all sharp, uniformly curved ventrally. Mouthhooks are strongly sclerotized, intermediate sclerite, dorsal and ventral cornua much less so. Intermediate sclerite straight and wide. Dorsal and ventral cornua equal in width; ventral more weakly sclerotized bearing an elongated small “ closed ” window posteriorly. Indentation index 84. Female head. (Figs. 188, 189) Yellow, with only arista, ocellar tubercle and post gena posteriorly black; ov s and iv s on the yellow ground; fronto-orbital plate visible laterally; 2 ors, 2 ori; lunule of medium heigh, narrowing posteriorly, reaching the level between ori a and ori p; 1 st fl of medium size, ovate; gena medially 0.38 × as high as maximum height of eye. Thorax viewed from the side. (Fig. 191) Mostly yellow, with kepst in ventral two-thirds, mr ventro-posteriorly and anatg ventro-posteriorly black. Wing. (Fig. 190) Hyaline, with yellowish undertone; with veins brown; costa reaching M 1; last section of CuA 1 2.15 × as long as penultimate; calypter yellow, margin wide black, fringe yellowish-grey. Wing length 1.8 mm. Female genitalia. (Figs. 193 – 195) Spermathecae relatively large, 0.27 – 0.33 × as high as anterior part of oviscape. Proctiger generally cylindrical, weakly sclerotized in anterior half except sternite 10; pair of long setae located on the posterior margin between cerci. Cercus angulate, 0.26 × as long as proctiger; medium setae located laterally and subapically, brush of short thin setae located apically. Spermathecae unequal in size, brown, ovate, with narrow plate-shaped basal collar. Neck of spermatheca distinctly narrower than spermathecal base. Spermathecal duct weakly sclerotized; slightly wrinkled; slightly swollen near the neck of spermatheca. Distribution. Belgium, Great Britain, the Czech Republic, Denmark, Finland, France, Germany, Hungary, Italy, Lithuania, Slovakia, Poland, Russia, Sweden, Switzerland, North Korea (Papp & Černý 2017). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF07260FFF7BFB0F59733342.taxon	description	(Figs. 44 – 46, 196 – 203) Material examined. Ukraine: Kyiv Region: near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 24. vi. 2021 — pupation outside the mine, 5. vii. 2021 — imago, Yu. Guglya, ex Lapsana communis (1 ♂ 2 ♀); Kharkiv Region: Kharkiv, Velyka Danylivka, 50 ° 2 ' 43 " N, 36 ° 19 ' 54 " E, 19. vi. 2020 — pupation outside the mine, 1. vii. 2020 — imago, Yu. Guglya, ex Sonchus oleraceus (1 ♂ 2 ♀); near Mokhnach, 49 ° 44 ' N 36 ° 32 ' E, 11. vi. 2023 — pupation outside the mine, 26 – 30. vi. 2023 — imago, Yu. Guglya, ex Lapsana communis (1 ♂ 2 ♀); near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 29. vi – 4. vii. 2020 — pupation outside the mine, 9 and 11. vii. 2020 — imago, Yu. Guglya, ex Lactuca serriola (2 ♂ 1 ♀). Hosts. Asteraceae: Cichorium intybus L., Crepis biennis L., C. capillaris (L.) Wallr., C. paludosa (L.) Moench, Lactuca virosa L., Sonchus arvensis L., S. asper (L.) Hill, S. oleraceus L. (Papp & Černý 2017). Lapsana communis L. (Guglya 2023). Mine. (Fig. 44) The solitary larva forms a greenish-white upper surface blotch leaf mine. Pupation takes place outside the mine. Puparium. (Figs. 45, 46) Yellow, glossy, 2.0 mm long, with distinct segmentation; surface quite smooth. Posterior spiracles brown, set on yellow conical protuberances entirely strongly separate; with four hook-like sessile bulbs set in a circular configuration. Anal plate scarcely protruding above the surface of the puparium viewed from the side and directed ventrally. Two small conical appendages developed on the sides of the anal plate viewed from the side and posteriorly. Cephalopharingeal skeleton. (Fig. 200) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute and abducted ventro-anteriorly. Both mouthhooks bear two large accessory teeth; dorsal are sharp, uniformly curved ventrally; ventral are blunt. Mouthhooks are strongly sclerotized; intermediate sclerite, dorsal and ventral cornua much less so. Intermediate sclerite straight and wide, gradually narrowing posteriorly. Dorsal cornu slightly wider than ventral ones; ventral cornu bears a long “ closed ” window posteriorly. Indentation index 95. Female head. (Figs. 196, 197) Yellow, with only arista, ocellar tubercle and post gena dorsally black; iv s on the yellow ground, ov s between yellow and black; fronto-orbital plate visible laterally; 2 ors, 2 ori; lunule rather high, narrowing posteriorly, reaching the level between ori a and ori p; 1 st fl of medium size, spherical; gena medially 0.26 × as high as maximum height of eye. Thorax viewed from the side. (Fig. 199) Moustly yellow, with kepst in ventral two-thirds, mr medially black; pprn dorsally, anepist in anterior half, anepm medially bear elongated blackish spots. Wing. (Fig. 198) Hyaline, with pale grey undertone; with beige veins; costa reaching M 1; last section of CuA 1 2.16 × as long as penultimate; calypter yellowish-grey, margin black and fringe grey. Wing length 1.8 mm. Female genitalia. (Figs. 201 – 203) Spermathecae relatively large, 0.27 × as high as anterior part of oviscape. Proctiger with relatively short and wide posterior two-half; dramatically narrowing anteriorly; two pairs of medium setae located on the posterior margin between cerci; addition several pairs of short and medium setae located along posterior margin. Cercus wide, oval, 0.36 × as long as proctiger; medium setae located laterally and subapically, brush of short thin setae located apically. Spermathecae equal, dark brown, spherical, with narrow irregular plate-shaped basal collar. Neck of spermatheca distinctly narrower than spermathecal base. Spermathecal duct weakly sclerotized; distinctly swollen near the neck of spermatheca. Distribution. The Czech Republic, France, Germany, Greece, Hungary, Lithuania, Malta, Spain, Canada, USA (Papp & Černý 2017). Ukraine (first record). Comments. Presence of this species in USA and Canada needs confirmation, beacause only females presumably of this species were collected there. Owen Lonsdale studied American specimens and didn’t include L. endiviae in the list of Agromyzidae of North America (Lonsdale 2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF05260EFF7BFC275BF532B6.taxon	description	(Figs. 47 – 50, 204 – 210) Material examined. Ukraine: Volyn Region: near Shatsk, 51 ° 28 ' 28 " N, 23 ° 48 ' 27 " E, 23. v. 2019, Yu. Guglya (1 ♂); Transcarpathia: Uzhhorod, Botanical Garden, 48 ° 37 ' N, 22 ° 18 ' E, 4. vii. 2019, Yu. Guglya (3 ♂ 1 ♀); near Nevytske, 48 ° 41 ' N, 22 ° 24 ' E, 17. vii. 2017, Yu. Guglya (1 ♀); 1 km E Rakhiv Center, 830 m a. s. l., 48 ° 03 ' N, 24 ° 13 ' E, 21 vii. 2017, Yu. Guglya (1 ♀); 1 km SEE Rakhiv, 730 m a. s. l., 48 ° 03 ' N, 24 ° 13 ' E, 22. vii. 2017, Yu. Guglya (1 ♂); Vinnytsa Region: near Dochno, 48 ° 16 ’ N, 29 ° 18 ’ E, 2. vii. 2019, Yu. Guglya (1 ♂); K yiv Region: Rzhyshchiv, deciduous forest, 49 ° 58 ' 25 " N, 31 ° 06 ' 20 " E, 23. vii. 2021 — pupation outside the mine, 4. viii. 2021 — imago, Yu. Guglya, ex Galium odoratum (1 ♂ 3 puparia); Rzhyshchiv, 49 º 57 ' 41 " N, 31 º 07 ' 34 " E, 23. vii. 2021, Yu. Guglya (1 ♀); S Rzhyshchiv, 49 º 56 ' 13 " N, 31 º 02 ' 46 " E, 20. vii. 2021, Yu. Guglya (1 ♀); Kharkiv Region: near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 7. vi. 2020, Yu. Guglya (1 ♀); near Rubizhne, 50 ° 07 ' N, 36 ° 46 ' E, 19. vii. 2014, Yu. Guglya (2 ♂). Hosts. Rubiaceae: Galium boreale L., G. triflorum Michx., G. rubioides (syn. broterianum Boiss & Reut., valantioides M. Bieb., G. verum L. and G. boreale L.), G. rotundifolium L. (Spencer 1969), G. asprellum Michx., circaezans Michx. Diodia virginiana L. (Eiseman & Lonsdale 2018), Galium odoratum (L.) Scop. — a newly recorded host plant. Mine. (Fig. 47) The solitary larva forms a white upper surface linear leaf mine. Pupation takes place outside the mine. Puparium. (Figs. 49, 50) Bright yellow, glossy, with brown posterior spiracles, 1.8 – 2.0 mm long, with distinct segmentation; surface quite smooth. Posterior spiracles set on yellow conical protuberances entirely strongly separate; with four hook-like sessile bulbs set in a circular configuration. Anal plate scarcely protruding above the surface of the puparium viewed from the side; directed ventrally. Two round ventral tubercles developed between the anal plate and posterior spiracles viewed from the side and posteriorly. Cephalopharingeal skeleton. (Fig. 207 a, b) Mouthhooks equal in size, with ventro-anterior portion wide; right rounded and abducted anteriorly, left flattened and abducted ventro-anteriorly. Both mouthhooks bear two rather large accessory teeth; left ventral tooth is less acute. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal and ventral cornua much less so. Intermediate sclerite convex dorsally. Indentation index 89. Female head. (Figs. 204, 205) Yellowish-brown, with contrasting yellow 1 st fl; fronto-orbital plate not visible laterally (as in Fig. 48); 2 ors, 2 – 3 ori (ocasionaly slender ori a present); 1 st fl of medium size, round. Gena wider at rear, in widest part 0.33 × as wide as maximum height of eye. Vibrissa slender, 0.28 × as long as maximum height of eye. Wing. (Fig. 206) Hyaline, with pale beige undertone; with brown veins; costa reaching M 1; last section of CuA 1 2.25 × as long as penultimate; calypter, margin and fringe grey. Wing length 2.0 mm. Female genitalia. (Figs. 207 – 209) Spermathecae relatively large, 0.27 × as high as anterior part of oviscape. Proctiger generally cylindrical, gradually widening posteriorly; pair of long setae located on the posterior margin between cerci. Cercus oval, 0.36 × as long as proctiger; medium setae located laterally and subapically, brush of short thin setae located apically. Spermathecae equal in size, brown, spherical, with narrow rim-shaped basal collar. Spermathecal duct weakly sclerotized; slightly wrinkled; swollen near the capsule of spermatheca. Distribution. Belgium, Czech Republic, France (Corsica), Germany, Greece, Hungary, Italy, Lithuania, Poland, Slovakia and Switzerland (Papp & Černý 2017), Canada, USA (Lonsdale 2021). Ukraine (first record). Comments. Spencer (1969) provide Galium rubioides without species authorship as a host of Praspedomyza galiivora sp. n. which later was transferred to the genus Liriomyza Mik by Lonsdale (2017). According POWO (2019) G. rubioides L. is a valid species, G. rubioides Brot is a synonym of G. broterianum Boiss & Reut., G. rubioides Pollich ia a synonym of G. valantioides M. Bieb., G. rubioides Suter is a synonym of G. verum L. and finally G. rubioides Willd. ex Ledeb is a synonym of G. boreale L. So, it’s hard to say what species of host exactly meant Kennet A. Spencer.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF04260EFF7BFD735B02342A.taxon	materials_examined	Material examined. Ukraine: Kharkiv Region: near Mokhnach, 49 ° 44 ' N 36 ° 32 ' E, 12. vi. 2023 — pupation outside the mine, 25 – 26. vi. 2023 — imago, Yu. Guglya, ex Lactuca chaixii (1 ♂ 1 ♀); same locality, 12. vi. 2023 — pupation outside the mine, 24. vi. 2023 — imago, Yu. Guglya, ex Lamium purpureum (1 ♂); same locality, 12. vi. 2023 — pupation outside the mine, 28 – 29. vi. 2023 — imago, Yu. Guglya, ex Lapsana communis (1 ♂ 1 ♀); near Haidary, 49 ° 37 ' N, 36 ° 19 ' E, 27. vii. 2020 — pupation outside the mine, 8. viii. 2020 — imago, Yu. Guglya, ex Lamium maculatum (1 ♀). Comments. Hosts, mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF04260EFF7BFB0F5AA83582.taxon	materials_examined	Material examined. Ukraine: Kyiv Region: near Rzhyshchiv, near ecostation “ Hlyboki Balyky ”, 50 ° 04 ' 33 " N, 31 ° 15 ' 29 " E, 25. vi. 2021 — pupation outside the mine, 15. x. 2021 — dead imago found, Yu. Guglya, ex Solidago virgaurea (2 ♂ 1 ♀); Kharkiv Region: near Haidary, 49 ° 37 ' N, 36 ° 19 ' E, 25. vii. 2020 — mines with larvae collected, Yu. Guglya, ex Solidago virgaurea (2 puparia). Comment. Mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF042608FF7BFA665E4C339E.taxon	description	(Figs. 51 – 54, 211 – 218) Material examined. Ukraine: Transcarpathia: 4 km W Yasynia, 860 m a. s. l., 48 ° 14 ' 13 " N, 24 ° 16 ' 39 " E, 6. viii. 2021 — mines with larvae collected, 18 – 20. viii. 2021 — pupation outside the mine, 21. ii. 2022 — imago, Yu. Guglya, ex Aconitum sp. (2 ♀ 1 puparia). Hosts. Ranunculaceae: Aconitum L., Delphinium Tourn. ex L. (Benavent-Corai et al. 2015). Mine. (Fig. 51, 52) Several larvae form a gregarious brown blotch leaf mine distinctly visible from both leaf sides. Pupation takes place either within or outside the mine. Puparium. (Figs. 53, 54) Orange, matt, 3.0 mm long, with deep segmentation; surface uniformly covered with numerous fine brown spines. Also with a row of sparse larger spines encircling each segment medially. Posterior spiracles set on stout, separated conical protuberances; orange, with more than 20 minute brown spherical sessile bulbs in a kidney-shaped configuration arranged around group of several slightly larger bulbs. Cephalopharingeal skeleton. (Fig. 214 a, b) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute and abducted ventrally. Mouthhooks bear two large accessory teeth; all sharp, uniformly curved ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal and ventral cornua very weakly so. Dorsal cornu slightly wider and more sclerotized than ventral ones. Indentation index 83. Female head. (Figs. 211, 212) Pale yellow, with post gena and antenna dark brown, ocellar tubercle and palpi black; fronto-orbital plate not visible laterally; 2 ors, 2 ori. Gena wider at rear, in widest part 0.48 × as wide as maximum height of eye. Vibrissa long, straight, 0.48 × as long as maximum height of eye. Wing. (Fig. 213) Hyaline, with greyish undertone; with beige veins; costa reaching R 3 + 4; last section of CuA 1 7.5 × as long as penultimate; second cross vein absent; calypter and margin pale yellow, fringe grey. Wing length 3.0 mm. Female genitalia. (Figs. 215 – 218) Proctiger generally cylindrical, slightly expanding posteriorly. Two pairs of long setae located on posterior margin between cerci. Cercus oval, 0.29 × as long as length of proctiger; long setae located subapically, brush of short setae located apically. Spermathecae subequal in size, brown, with wrinkled surface, slightly wider than high and flattened basally and apically. Internal duct invagination 0.73 – 0.77 × as deep as height of spermatheca, slightly tapering apically. Spermathecal duct narrow and very weakly sclerotized. Ventral receptacle brown, S-shaped. Body of receptacle large, ovate, with an oblique opening, 0.42 × as wide as length of receptacle. Basal connecting tube rather wide, curved, 3.75 × as wide as tail of receptacle, which apically bears 2 weakly sclerotized wide flattened blades. Distribution. Holarctic species (Papp & Černý 2019). Ukraine (first record). Comments. The collected series consisted of nine larvae, of which one pupated within the mine and eight outside. Five parasitoids wasps (Braconidae) were also reared from this series.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF02260BFF7BFC5A5AB633BA.taxon	description	(Figs. 55 – 58, 219 – 226) Material examined. Ukraine: Kyiv Region: S Rzhyshchiv, 49 ° 56 ' 07 " N, 31 ° 02 ' 50 " E, 25. vi. 2021 — mines with puparia collected, 4 and 6. vii. 2021 — imago, Yu. Guglya, ex Cornus sanguinea (2 ♀); near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 23. vii. 2021 — mines with puparia collected, 4 and 20. viii. 2021 — imago, Yu. Guglya, ex Cornus sanguinea (3 ♀). In addition empty mines on Cornus sanguinea were collected in Ukraine and preserved in the herbarium from: Transcarpathia: between Kamianytsa and Antalivska Polyana, 48 ° 40 ' 20 " N, 22 ° 25 ' 43 " E, 410 m a. s. l., 26. vi. 2018 — empty fresh mines collected, Yu. Guglya, ex Cornus sanguinea (4 mines); Vinnytsa Region: near Chechelnyk, 48 ° 10 ' N 29 ° 20 ' E, 1. vii. 2019 — empty fresh mine collected, Yu. Guglya, ex Cornus sanguinea (1 mine); Kyiv Region: near Ulianyky, 49 ° 54 ' 50 " N 31 ° 07 ' 28 " E, 20. vii. 2021 — empty fresh mine collected, Yu. Guglya, ex Cornus sanguinea (3 mines); near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 28. viii. 2021 — empty fresh mine collected, Yu. Guglya, ex Cornus sanguinea (1 mine); Kharkiv Region: Kharkiv, Botanical Garden, 50 ° 01 ' 46 " N 36 ° 14 ' 02 " E, 10. vi. 2012 — empty dried mine collected, Yu. Guglya, ex Cornus sanguinea (1 mine). Hosts. Cornaceae: Cornus sanguinea L. and others Cornus spp. (Spencer 1976). Mine. (Figs. 55, 56) The solitary larva forms a greenish, wide linear, upper surface leaf mine. Several larvae can feed in one leaf at the same time, but the mine do not merge and stay distinct. Pupation takes place either within or outside the mine. Also see comments below. Puparium. (Figs. 57,58) Orange, silky-shining, with black, glossy posterior spiracles, 1.8 mm long, with shallow but distinct segmentation; surface finely wrinkled except for wide bands of fine spines. Posterior spiracles set on short stout separate conical protuberances; with 11 – 14 fine black sessile bulbs set in a kidney-shaped configuration. Anal plate orange, scarcely protruding above the surface of the puparium viewed from the side and directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 222 a, b) Mouthhooks equal in size, with ventro-anterior portion acute and abducted anteriorly. Right mouthhook with rounded posterior margin; bears distinct acute dorsal tooth and scarcely expressed ventral tooth. Left mouthhook with flattened posterior margin; with two distinct acute teeth. Mouthhooks and intermediate sclerite ventrally are strongly sclerotized; intermediate sclerite dorsally and dorsal and ventral cornua very weakly so. Dorsal cornu more sclerotized in anterior third. Indentation index 84. Female head. (Figs. 219, 220) Brown, with gena and labellum orange, ocellar tubercle black; fronto-orbital plate not visible laterally; 2 ors, 1 – 2 ori. 1 st fl small, spherical, covered with thick pale pubescence. Gena wider at rear, in widest part 0.3 × as wide as maximum height of eye. Vibrissa long, straight, 0.46 × as long as maximum height of eye. Wing. (Fig. 221) Hyaline, with brown veins; costa ending slightly after R 3 + 4; second cross vein absent; second cross vein absent; calypter pale grey, margin and fringe black. Wing length 2.1 mm. Female genitalia. (Figs. 223 – 226) Spermathecae small, 0.08 × as high as anterior part of oviscape. Proctiger generally cylindrical, slightly expanding posteriorly. Pair of long setae located on posterior margin between cerci. Cercus oval, 0.23 × as long as proctiger; medium setae located subapically, brush of short setae located apically. Spermathecae subequal in size, brown, spherical. Spermathecal duct very weakly sclerotized; with cylindrical pigmented zone near the base of the capsule of spermatheca that is 0.5 × as long as height of spermatheca. Ventral receptacle brown, S-shaped. Body of receptacle large, spherical; flattened side with opening, 0.54 × as wide as length of receptacle. Basal connecting tube rather wide, slightly curved, 2.5 × as wide as tail of receptacle, tail apically with 2 weakly sclerotized narrow blades. Distribution. Belgium, Bulgaria, the Czech Republic, Denmark, France, Germany, Great Britain, Hungary, Lithuania, the Netherlands, Poland, Romania, Slovakia, Sweden, Kyrgyzstan, Canada, USA (Papp & Černý 2019). Ukraine (first record). Comments. Fresh mines (Fig. 55) are greenish due to a wide area of frass located centrally along the full length of the mine and occupying almost its entire width. In 2 – 3 weeks the mine and frass dry and become whitish (Fig. 56). In Ukraine the earliest dried mines were found in the beginning of June and later fresh mines were collected at the end of June, July and August in the same locality, suggesting that three or four generations developing during the year.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF012635FF7BFC7E5AB13206.taxon	description	(Figs. 59 – 61, 227 – 234) Material examined. Ukraine: Transcarpathia: 32 km NNE Tiachiv, 48 ° 17 ' N, 23 ° 43 ' E, 19. vii. 2017, Yu. Guglya (3 ♂); 2.3 km N Kvasy, 48 ° 10 ' 13 " N, 24 ° 18 ' 18 " E, 17. viii. 2021 — pupation outside the mine, Yu. Guglya, ex Astrantia major (1 ♂ 2 puparia); Ivano-Frankivsk Region: near Zarosliak, 1400 m a. s. l., 48 ° 10 ' 10 " N, 24 ° 31 ' 24 " E, 19 – 22. viii. 2021 — pupation outside the mine, 2 – 9. ix. 2021 — imago, Yu. Guglya, ex Astrantia major (9 ♂ 3 ♀). Hosts. Apiaceae: Astrantia major L. (Hendel 1924). Mine. (Fig. 59) Larvae form solitary or gregarious greenish blotch upper surface leaf mines. Pupation takes place outside the mine. Puparium. (Figs. 60, 61) Blackish-brown, glossy, 2.0 mm long, with distinct but shallow segmentation; surface smooth, except for wide bands of fine spines. Posterior spiracles set on wide cylindrical protuberances that are entirely separate; black, glossy, with 12 – 17 sessile bulbs arranged in a narrow open ellipse. Anal plate slightly protruding above the surface of the puparium viewed from the side; directed ventrally. Cephalopharingeal skeleton. (Fig. 230) Right mouthhook larger dorsally than the left, with ventro-anterior portion acute and abducted ventro-anteriorly. Mouthhooks widened ventrally, bearing two finger-shaped teeth. Mouthhooks and intermediate sclerite ventrally and posteriorly are strongly sclerotized; intermediate sclerite dorso-anteriorly and dorsal cornu in anterior third and posterior third weakly so. Indentation index 90. Female head. (Figs. 227, 228) Dull yellow, ocellar tubercle brown; fronto-orbital plate not visible laterally; 2 ors, 1 ori; posterior ors the smallest, anterior ors the longest, ori of medium length; orbital setulae sparse, elongate, proclinate. Lunule very wide and low, scarcely reaching the level of ori. 1 st fl large, spherical, covered with thick pale pubescence. Gena wider at rear, in widest part 0.43 × as wide as maximum height of eye. Vibrissa of medium length, straight, 0.3 × as long as maximum height of eye. Wing. (Fig. 229) Hyaline, with beige veins; costa reaching R 3 + 4; second cross vein absent; second cross vein absent; calypter pale greyish-beige, margin and fringe black. Wing length 2.1 mm. Female genitalia. (Figs. 231 – 234) Spermathecae of medium size, 0.16 × as high as height of anterior part of oviscape. Proctiger cylindrical, with two pairs of medium setae located on posterior margin between cerci. Cercus short and broadly rounded, 0.22 × as long as proctiger; medium setae located subapically, several short setae located apically. Spermathecae unequal in size, brown, with wrinkled surface, wider than high and flattened basally and apically. Largest spermatheca tapering apically, smallest much less so. Internal duct invagination 0.63 – 0.69 × as deep as height of spermatheca, slightly tapering apically. Spermathecal duct narrow and very weakly sclerotized. Ventral receptacle brown, compressed S-shaped, with weakly sclerotized tail. Body of receptacle spherical, with slightly curved basal connecting tube, strongly sclerotized. Opening located on narrow projection, 0.7 × as wide as a diameter of spherical part of body. Distribution. The Czech Republic, Germany, Great Britain, Poland, Turkey (Papp & Černý 2019). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF3F2637FF7BFDE25E4731CE.taxon	description	(Figs. 62 – 64, 235 – 241) Material examined. Ukraine: Transcarpathia: near Nevytske, 48 ° 42 ' N, 22 ° 26 ' E, 14. vii. 2017, Yu. Guglya (1 ♂); Kvasy, 560 m a. s. l., 48 ° 09 ' 52 " N, 24 ° 17 ' 03 " E, 12. viii. 2021 — mines with puparia collected, 14 – 16. viii. 2021 — pupation outside the mine, 30. viii – 1. ix. 2021 — imago, Yu. Guglya, ex Chaerophyllum aromaticum (1 ♂ 3 ♀ 3 puparia); same locality, 12. viii. 2021, Yu. Guglya (1 ♀); near Shyrokyi Luh, 48 ° 17 ' N, 24 ° 43 ' E, 19. vii. 2017, Yu. Guglya (1 ♂). Mine. (Fig. 62) The solitary larva forms a whitish linear upper surface leaf mine. Pupation takes place outside the mine. Puparium. (Figs. 63, 64) Brown, semi-glossy, 2.0 mm long, with shallow but distinctly visible segmentation; surface quite smooth except for narrow bands of fine spines. Posterior spiracles set flat on the body cuticle and entirely separate; black with 17 – 19 fine sessile bulbs in an elliptical configuration. Ventral portion of last abdominal segment not protruding posteriorly viewed from the side. Anal plate directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 238) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventrally. Mouthhooks with two accessory teeth; dorsal tooth sharp, uniformly curved and directed ventrally; ventral rounded directed anteriorly. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal cornu much less so. Ventral cornu very weakly sclerotized, bearing large “ closed ” window posteriorly. Intermediate sclerite straight, uniformly widening posteriorly, bearing sharp tooth on the posterior third of ventral margin that is directed ventro-anteriorly. Indentation index 82. Female head. (Figs. 235, 236) Brown, with dark yellow labellum and black 1 st fl with thick brown pubescence; fronto-orbital plate not or only slightly visible laterally; 2 ors, 1 ori; ori and ors a distinctly longer than ors p; lunule of medium height, broad, semicircular, reaching the level of ori; 1 st fl large, rounded apically; gena medially 0.24 × as high as maximum height of eye. Wing. (Fig. 237) Hyaline, with greyish undertone; veins beige; costa ending after R 3 + 4; second cross vein absent; calypter pale beige, margin and fringe brown. Wing length 2.8 mm. Female genitalia. (Figs. 239 – 241) Spermathecae of medium size, 0.21 × as high as anterior part of oviscape. Proctiger barrel-shaped, with several long setae located on posterior margin between cerci. Cerci spaced far from each other. Cercus wide oval, 0.29 × as long as proctiger; medium setae located apically and along inner surface. Spermathecae equal in size, brown, with wrinkled surface, wider than high and flattened basally. Internal duct invagination 0.76 × as deep as height of spermatheca, cylindrical. Spermathecal duct narrow and very weakly sclerotized. Ventral receptacle brown, S-shaped, with weakly sclerotized tail that is bowl-shaped in basal half. Body of receptacle spherical, with slightly curved basal connecting tube, strongly sclerotized. Basal connecting tube at confluence of tail flat, 1.75 × as wide as tail. Opening of medium size, 0.7 × as wide as a diameter of spherical part of body. Comments. The male genitalia of four species occuring in Ukraine — Phytomyza adjuncta, P. aurei, P. melana and P. vilnensis are highly similar and can be confused in the absence of comparative material. Additionally, adults can be captured together and larvae of all four form similar linear leaf mines on Apiaceae (P. adjuncta in Pimpinella spp., the remaining three species in Chaerophyllum aromaticum). The puparia of P. aurei and P. melana are also identical. In Guglya (2021), reared specimens of Phytomyza melana were incorrectly diagnosed as P. aurei. Additional subsequent observation of reared and captured males and females managed to correct the mistake. Figs. 189 – 193, 561 – 566 in Guglya (2021) relate to P. melana; correctly reidentified material of P. aurei and P. melana are listed here in the relevant species entries. Male captured in Nova Stuzhytsa identified as P. aurei in Guglya (2021) has been reidentified as P. vilnensis. Hosts and distribution see Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF3D2637FF7BFDAA5E7236FE.taxon	description	(Figs. 65 – 67, 242 – 248) Material examined. Ukraine: Kharkiv Region: N Kharkiv, Piatykhatky, 50 ° 05 ' 42 " N, 36 ° 15 ' 38 " E, 10. vii. 2021, Yu. Guglya (1 ♂); near Petrivske, 49 ° 10 ’ N, 36 ° 58 ’ E, 28. vi. 2020 — mine with larva collected, 30. vi. 2020 — pupation outside the mine on lower leaf surface, 11. vii. 2020 — imago, Yu. Guglya, ex Echinops sphaerocephalus (1 ♀). Hosts. Asteraceae: Echinops spp. (Spencer 1976). Mine. (Fig. 65) The solitary larva forms a yellowish linear upper surface leaf mine, with frass arranged in long lines along the margin. Pupation takes place outside the mine. Puparium. (Figs. 66, 67) Brown, semi-glossy, 1.9 mm long, with distinct segmentation; surface quite smooth except for wide bands of fine spines between all segments. Posterior spiracles set flat on the body cuticle and entirely separate; black with 12 fine sessile bulbs in an elliptical configuration. Ventral portion of last abdominal segment slightly protruding posteriorly viewed from the side. Anal plate directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 245) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventrally. Mouthhooks bear two accessory teeth; both dorsal and right medial sharp, uniformly curved and directed ventrally; left ventral rounded directed anteriorly. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal cornu much less so. Ventral cornu bearsa small “ closed ” window medially. Intermediate sclerite straight, bearing two sharp teeth on the posterior third of ventral margin that are directed ventro-posteriorly. Indentation index 80. Female head. (Figs. 242, 243) Pale orange, not bright, with ped and 1 st fl black; orbit not or scarcely projecting above eye in profile; 2 ors, 1 ori; lunule small, narrow, semicircular, reaching the level of ori; 1 st fl large, rounded apically, with thick gold pubescence; gena medially 0.32 × as high as maximum height of eye. Wing. (Fig. 244) Hyaline, with beige veins; costa ending after R 3 + 4; second cross vein absent; calypter and margin pale yellow, fringe black. Wing length 2.1 mm. Female genitalia. (Figs. 246 – 248) Spermathecae of medium size, 0.15 × as high as anterior part of oviscape. Proctiger wide, cylindrical, with pair of medium setae located on posterior margin between cerci. Cercus broadly ovate, 0.46 × as long as proctiger; several thin elongate setae and thick short setae located subapically and apically. Spermathecae equal in size, brown, wider than high and flattened basally and apically. Internal duct invagination wrinkled, 0.8 × as deep as height of spermatheca, tapering apically. Spermathecal duct relatively wide and very weakly sclerotized. Ventral receptacle brown, S-shaped, with weaker sclerotized tail that is two-bladed basally; body of receptacle spherical, with slightly curved basal connecting tube, strongly sclerotized; opening relatively small, 0.57 × as wide as a diameter of spherical part of body. Distribution. Belgium, the Czech Republic, Germany, Great Britain, Hungary, Italy, Lithuania, Norway, Poland, Sweden, Switzerland, Turkey, Serbia (Papp & Černý 2019). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF3D2636FF7BF8BA5EA63632.taxon	description	(Figs. 68 – 70, 249 – 255) Material examined. Ukraine: Volyn Region: Ostrovje, 51 ° 33 ' 06 " N, 23 ° 47 ' 24 " E, 24. v. 2019, Yu. Guglya (1 ♂); Transcarpathia: Kvasy, 560 m a. s. l., 48 ° 09 ' 52 " N, 24 ° 17 ' 03 " E, 12. viii. 2021, Yu. Guglya (1 ♂); 1 km SEE Rakhiv Center, 730 m a. s. l., 48 ° 3 ' N, 24 ° 13 ' E, 22. vii. 2017, Yu. Guglya (1 ♂ 2 ♀); Kyiv Region: near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 23. v. 2021 — mine with larva collected, 7. vi. 2021 — imago, Yu. Guglya, ex Anthriscus sylvestris (1 ♂); same locality, 24. vi. 2021 – pupation outside the mine, 1. x. 2021 — imago, Yu. Guglya, ex Anthriscus sylvestris (1 ♂); Kharkiv Region: near Mokhnach, 49 ° 44 ' N 36 ° 32 ' E, 10. vi. 2023, Yu. Guglya (6 ♂ 7 ♀); Donetsk Region: Kryva Luka, 48 ° 52 ' 34 " N, 37 ° 54 ' 37 " E, 1. vi. 2019, Yu. Guglya (1 ♂). Hosts. Apiaceae: Anthriscus Pers., Carum L., Chaerophyllum L., Conium L., Conopodium W. D. J. Koch, Daucus L., Orlaya Hoffm., Selinum L., Sison L., Tordylium Tourn. ex L., Torilis Adans. (Benavent-Corai et al. 2005). Mine. (Fig. 68) The solitary larva forms a white linear upper surface leaf mine, moving along the leaf margin. Frass grains arranged along both margins of the mine irregularly. Pupation takes place outside the mine. Puparium. (Figs. 69, 70) Pale brown, semi-glossy, 1.8 mm long, with shallow but distinctly visible segmentation; surface quite smooth except for wide bands of fine spines between all segments. Posterior spiracles set flat on the body cuticle and entirely separate; dark brown with 15 – 17 fine sessile bulbs in an elliptical configuration. Ventral portion of last abdominal segment not protruding posteriorly viewed from the side. Anal plate directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 252) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventrally. Mouthhooks bear two sharp accessory teeth; dorsal teeth are larger than ventral; all uniformly curved and directed ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal and ventral cornua much less so. Intermediate sclerite straight, long, 1.47 × as long as height of right mouthhook; bears two sharp teeth on the posterior third of ventral margin directed ventro-posteriorly. Ventral cornu bears small “ closed ” window posteriorly. Indentation index 84. Female head. (Figs. 249, 250) Brown, with frons, face, gena and labellum brownish-yellow; 1 st fl with thick pale pubescence; fronto-orbital plate visible laterally; 2 ors, 1 ori; lunule of medium height, broad, semicircular, reaching the level of ori; 1 st fl large and spherical, with venter somewhat flattened; gena medially 0.31 × as high as maximum height of eye. Wing. (Fig. 251) Hyaline, with costa, R 1 + 2, R 3 + 4 brown, the remaining veins pale; costa ending after R 3 + 4; second cross vein absent; calypter beige, margin and fringe brown. Wing length 1.9 mm. Female genitalia. (Figs. 253 – 255) Spermathecae of medium size, 0.2 × as high as anterior part of oviscape. Proctiger cylindrical, with two pairs long setae located on posterior margin between cerci. Cerci closely spaced. Cercus wide oval, tapering apically, 0.2 × as long as proctiger; medium and short setae located subapically and apically. Spermathecae equal in size, brown, with smooth surface, wider than high and flattened basally and apically. Internal duct invagination wrinkled, 0.85 × as deep as height of spermatheca, cylindrical. Spermathecal duct narrow and very weakly sclerotized. Ventral receptacle brown, S-shaped, with well sclerotized tail that is bowl-shaped in basal half. Body of receptacle spherical, with slightly curved basal connecting tube, strongly sclerotized. Basal connecting tube at confluence of tail invaginated, 2.2 × as wide as tail. Opening of medium size, 0.67 × as wide as a diameter of spherical part of the body. Distribution. Palaearctic species known from 24 European countries, as well as from Turkey and Japan (Papp & Černý 2019). Ukraine (first record). Comments. At least two generations develop during the year in Ukraine, with pupation at the end of May and at the end of June. Female genitalia are very similar to P. aurei. The main differentiating characteristics are the shape of proctiger, cerci and basal connecting tube with tail in ventral receptacle (see description above).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF3C2633FF7BF9F6587830CA.taxon	description	(Figs. 71 – 73, 256 – 265) Material examined. Ukraine: Transcarpathia: near Lukh, 47 ° 56 ' 20 " N 24 ° 06 ' 40 " E, 12 – 15. v. 2018, Yu. Guglya, ex Doronicum austriacum (3 puparia); Ivano-Frankivsk Region: near Zarosliak, 1400 m a. s. l., 48 ° 10 ' 10 " N 24 ° 31 ' 24 " E, 19 – 21. viii. 2021 — pupation outside the mine, Yu. Guglya, ex Doronicum austriacum (2 puparia); foothills of Brescul Mt., 1370 m a. s. l., 48 ° 09 ' N 24 ° 31 ' E, 30 – 31. viii. 2022 — pupation outside the mine, 3. ii. 2023 — imago, Yu. Guglya, ex Doronicum austriacum (1 ♂ 3 puparia). In addition empty mines on Doronicum austriacum were collected in the Ukrainian Carpathians and preserved in the herbarium: Transcarpathia: 32 km NNE Tiachiv, 48 ° 17 ' N, 23 ° 43 ' E, 19. vii. 2017; “ Peremychka ” tourist parking, 1530 m a. s. l., 48 ° 09 ' 34 " N 24 ° 28 ' 06 " E, 16. viii. 2021; spruce-beech forest between “ Peremychka ” and “ Bilyi ”, 1400 m a. s. l., 48 ° 08 ' 29 " N 24 ° 28 ' 51 " E, 31. viii. 2018; spruce-beech forest near foothills of Turkul Mt., 1210 m a. s. l., 48 ° 06 ' 37 " N 24 ° 29 ' 59 " E, 01. ix. 2018; Ivano-Frankivsk Region: foothills of Pip Ivan Chornohorski Mt., 1340 m a. s. l., 48 ° 03 ' 24 " N 24 ° 35 ' 51 " E, 13. viii. 2019; foothills of Dantsyzh Mt., 1460 m a. s. l., 48 ° 08 ' 04 " N 24 ° 32 ' 52 " E, 3. ix. 2018; foothills of Turkul Mt., 1520 m a. s. l., 48 ° 07 ' 09 " N 24 ° 30 ' 27 " E, 24. viii. 2022. Hosts. Asteraceae: Doronicum austriacum Jacq., D. columna Tenore, D. pardalianches L. (Griffiths 1974), D. grandiflorum Lam. (Ellis 2017). Mine. (Fig. 71) The solitary larva forms a yellow linear upper surface leaf mine, with small grains of frass arranged along the mine margin. Pupation takes place outside the mine. Puparium. (Figs. 72, 73) Black, glossy, 2.8 mm long, with distinct segmentation; surface quite smooth except for narrow bands of fine spines between all segments. Posterior spiracles set flat on the body cuticle and entirely separate; black with 18 – 19 fine sessile bulbs in an elliptical configuration. Two small round appendages developed right above anal plate. Anal plate slightly protruding ventro-posteriorly viewed from the side. Larva described in Beiger (1973). Cephalopharingeal skeleton. (Fig. 260) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventro-anteriorly. Mouthhooks bear two sharp accessory teeth that are uniformly curved and directed ventrally; dorsal teeth are larger than ventral. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal and ventral cornua much less so. Intermediate sclerite slightly curved; with three sharp teeth on posterior third of ventral margin that are directed ventro-posteriorly. Dorsal cornu relatively wide, uniformly sclerotized; ventral cornu very weakly sclerotized. Indentation index 85. Male head. (Figs. 256, 257) Bicolour, with fronto-orbital plate, frons, gena, ventral face margin and labellum yellow, lunule dark yellow and pprn, ocellar tubercle, areolae of ors, antennae, face and palpi brown; iv s located between yellow and brown ground, ov s on brown ground; 1 st fl large, ovate, with long thick pale pubescence; fronto-orbital plate visible laterally; 2 ors, 1 ori; lunule of medium height, broad, semicircular, reaching the level of lower ors; gena medially 0.27 × as high as maximum height of eye. Wing. (Fig. 258) Hyaline with greyish undertone; costal vein ochraceous, ending on R 4 + 5; second cross vein absent; calypter dirty yellow, margin orange, fringe black. Wing length 3.0 mm. Mesonotum, scutellum. (Fig. 259) Mesonotum ash-grey, with a slightly shining viewed from above; spal s located on the border of grey and pale yellow ground; 1 + 3 dc s, strong, with setae becoming longer posteriorly; acr s elongate, arranged in 3 – 4 irregular rows. Scutellum ash-grey with pale yellow patches latero-anteriorly of the b sctl s. All femora, 2 nd and 3 rd tibiae and tarsi dark brown with wide pale yellow knees, 1 st tibia and tarsi yellowish-brown. Male genitalia. (Figs. 261 – 265) Epandrium subovate; hypandrium V-shaped, with narrow arms, 0.5 × as wide as width of pregonite. Pregonite rectangular, more weakly sclerotized, bearing two long strong setae near the inner basal corner viewed from below. Postgonite large, elongated, 3.0 × as long as its maximum width, with set at a distance widened lobe located apically. Ejaculatory apodeme small, with triangular, weakly sclerotized blade; base well sclerotized, narrow, bearing long and narrow finger-shaped appendage posteriorly. Basiphallus and hypophallus strongly sclerotized, distiphallus and paramesophalli weakly so. Basiphallus long, 2.8 × as long as distiphallus, with narrow asymmetrical waved lamellae; each lamellae bearing a group of small and medium spines anteriorly (right group not seen from below). Hypophallus arcuate, long viewed from below. Paramesophalli Y-shaped. Tubules of distiphallus widened and less sclerotized anteriorly viewed from the side and below. Male terminalia viewed from the side depicted in Beiger (1978) without verbal description. Distribution. Germany, Poland, Spain, Romania, Bulgaria, Slovenia, The Netherland (Ellis 2017). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF3C2633FF7BF9F6587830CA.taxon	discussion	Comments. This rare European species is common in the Ukrainian Carpathians where it concludes with its host plant Doronicum austriacum (own observations). The earliest empty mines were collected on 10 May and the latest occupied mines were collected on 27 August. Presumably, two or three generations develop during the year. The larva of P. doronici forms mines similar to mines of P. aronici described by Nowakowski (1962) from the Tatra Mountains. Both species develop in leaves of Doronicum species, and the morphology of puparia, wing, cephalopharyngeal skeleton and phallus shape are similar. Superficially P. aronici can be easily different from P. doronici in heaving 4 frontoorbital setae, black thorax, matt mesonotum, blackish-brown margin of calypter. Additionally only the 1 st knee is pale.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF392633FF7BFEAE587135DE.taxon	description	(Figs. 78 – 80, 266 – 271) Material examined. Ukraine: Vinnytsa Region: near Chechelnyk, Vyshenka location, 48 ° 10 ' N, 29 ° 20 ' E, 1. vii. 2019, Yu. Guglya (1 ♂); Kharkiv Region: near Petrivske, 49 ° 10 ’ N, 36 ° 58 ’ E, 24. iv. 2018 — puparia inside the stem mine collected, summer 2018 — imago, Yu. Guglya, ex Urtica dioica (1 ♀ 6 puparia); same locality, 10. v. 2021, Yu. Guglya (3 ♂ 3 ♀); near Rubizhne, 50 ° 07 ' N, 36 ° 46 ' E, 2. v. 2021 — puparium inside the stem mine collected, Yu. Guglya, ex Urtica dioica (1 puparium). Hosts. Urticacea: Urtica L. (Benavent-Corai et al. 2005). Mine. The larva feeds as an internal stem borer. Pupation takes place inside the stem. Puparium. (Figs. 78 – 80) Yellow, transparent, glossy, 3.8 mm long, with distinct segmentation; surface quite smooth. Posterior spiracles set on stout separate conical protuberances with 36 fine sessile bulbs in a double circular configuration. Anal plate distinctly protruding posteriorly viewed from the side. Cephalopharingeal skeleton. (Fig. 269) Mouthhooks equal in size, which appearing bilobate with one long stout tooth dorsally. Mouthhooks, intermediate sclerite and dorsal cornu are strongly sclerotized, ventral cornu much less so. Intermediate sclerite straight; dramatically tapering apically in anterior half. Ventral cornu bears distinct “ closed ” window posteriorly. Indentation index 84. Female head. (Figs. 266, 267) Mostly bright yellow, with ocellar tubercle and vertex black; i vt s and o vt s on black ground; 1 st fl with thick pale, elongated pubescence; fronto-orbital plate distinctly visible laterally; fronto-orbital plate and parafacial extremely wide; 2 ors, 2 ori, ori a very short; lunule low, broad, flattened dorsally, reaching the level between ori a and ori p; gena and parafacial together equal to the maximum vertical height of eye. Wing. (Fig. 268) Yellowish, with pale veins; costa ending after R 3 + 4; second cross vein absent; calypter yellow, margin and fringe darker. Wing length 3.0 mm. Thorax viewed from the side. (Fig. 268) Mostly yellow, with kepst in ventral half, mr ventro-posteriorly and ktg posteriorly black; anepst ventrally and anepm medially with greyish patches. Legs yellow. Female genitalia. (Figs. 270, 271) Spermathecae small, 0.06 × as high as anterior part of oviscape. Spermathecae equal in size, pale brown, spherical. Spermathecal duct narrow and very weakly sclerotized. Ventral receptacle brown, S-shaped, with weakly sclerotized tail. Body of receptacle spherical, with acutely curved basal connecting tube, strongly sclerotized. Opening small, located on tapering projection, 0.41 × as wide as a diameter of spherical part of the body. Distribution. A Holarctic species, recorded from 23 European countries, the United States and Canada (Papp & Černý, 2019). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF39263DFF7BF99A584033BA.taxon	description	(Figs. 74 – 77, 272 – 277) Material examined. Ukraine: Kharkiv Region: Kharkiv, Velyka Danylivka, 50 ° 01 ' N, 36 ° 18 ' E, 06. viii. 2024 — mines with larva collected, 15. viii. 2024 — imago, M. Fandikova, ex Clematis terniflora var. mandshurica (1 ♂ 1 puparium). Hosts. Ranunculaceae: Helleborus Toum. ex L. (Benavent-Corai et al. 2005), Clematis sp. (Guglya 2021) Clematis terniflora DC, C. integrifolia L. — a newly recorded host plant. Wing. (Fig. 272) Hyaline, greyish, with costa, R 1 + 2 and R 3 + 4 brown, with remaining veins paler; costa reaching R 3 + 4; second cross vein absent; calypter pale yellow, margin and fringe brownish. Wing length 2.5 mm. Male genitalia. (Figs. 273 – 276) Postgonite relatively narrow, 3.7 × as long as maximum width; bilobate laterally, with one lobe narrow, finger-shaped, and another lobe wide, rounded; set at a distance hook-shaped lobe located apically. Other features described and depicted in Papp & Černý (2019). Female genitalia. (Fig. 277) Proctiger generally cylindrical, weakly sclerotized in anterior half except for stronger sternite 10 and narrow dorsomedial area of tergite 10; four pairs of long setae located on the posterior margin between cerci. Cercus oval, 0.38 × as long as proctiger; short and medium setae spaced laterally, subapically and apically. Distribution. Palaearctic region (Guglya 2021), Nearctic region: Canada, USA (Eiseman et al. 2024). Comments. Adults of Phytomyza hellebori have been reared three times from two species of Clematis in Kharkiv (Ukraine): in 2013 (Fig. 75) and 2019 from C. integrifolia and in 2024 from C. terniflora. All mines were collected in one garden, where an unmined Helleborus sp. was also cultivated. In Ukraine, individuals of both plant host genera are commonly found, although hellebores are not found in the wild towards the northeast. In localities where both occur, it should be further investigated as to whether adult females are also preferentially ovipositing on Clematis. Male genitalia of P. hellebori are close to those of P. anemones Hering and P. fallaciosa Brischke. Paired sclerites of the basal part of the distiphallus in reared specimens from Clematis spp. are various (Figs. 273 – 275). The main differences among these species are external features, phenology, voltinism, specialities of the mining and host specialization. P. anemones and P. fallaciosa make apical mines, blotch and secondary blotch respectively, and P. hellebori make not apical secondary blotch mine. According to Spencer (1976) P. hellebori was reared from Helleborus spp. and Anemone hortensis; L. P. anemones from Anemone nemorosa L. and Clematis flammula L.; and P. fallaciosa — from Ranunculus spp. Taking into account all the above P. anemones clematobia reared from Clematis flammula emerged month later than known P. anemones (Hering 1967) and presumably it may turn out P. hellebori, but the correct status of this subspecies still needs clarification. Mine in Clematis sp. leaf, puparium, cephalopharingeal skeleton and female genitalia except proctiger depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF37263DFF7BFC7E591035DE.taxon	description	(Figs. 28 – 29, 178) Material examined. Ukraine: Transcarpathia: between Antalivtsy and Nevytske, road to Antalivska Poliana, 600 m a. s. l., 48 ° 40 ' N, 22 ° 24 ' E, 05. v. 2018, Yu. Guglya (1 ♂); Kyiv Region: near Rzhyshchiv, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 24. vii. 2021 — mines with puparia collected, Yu. Guglya, ex Lycopus europaeus (2 puparia); Kharkiv Region: Pisochyn, the River Merla Bank, 49 ° 57 ' 58 " N, 36 ° 05 ' 13 " E, 29. vi. 2024 – mine with puparium collected, Yu. Guglya, ex Lycopus europaeus (1 puparium). Female genitalia. (Fig. 178) Proctiger generally cylindrical, scarcely expanding posteriorly. Two pairs of long setae located on posterior margin between cerci. Cerci distinctly spaced. Cercus egg-shaped, 0.26 × as long as proctiger; long setae located subapically and laterally, brush of 4 – 5 short setae located apically. Comments. See above under Amauromyza rameli. Mine (Figs. 28 – 29), puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF37263CFF7BF99A5B933196.taxon	description	(Figs. 179, 180) Material examined. Ukraine: Transcarpathia: near Nevytske, 48 ° 41 ' N, 22 ° 25 ' E, 14. vii. 2017, Yu. Guglya (1 ♂); 1 km E Rakhiv Center, 830 m a. s. l., 48 ° 03 ' N, 24 ° 13 ' E, 21. vii. 2017, Yu. Guglya (1 ♀); Uzhhorod, 48 ° 37 ' N, 22 ° 17 ' E, 19. ix. 2017 — mines with larvae collected, 9. x. 2017 — imago, Yu. Guglya, ex Chaerophyllum aromaticum (1 ♂ 2 ♀); near Uzhhorod, “ Skalka ”, 48 ° 41 ' N, 22 ° 24 ' E, 17. ix. 2016 — mines with larvae collected, 11 – 13. iii. 2017 — imago, Yu. Guglya, ex Chaerophyllum aromaticum (2 ♀); Kvasy, 560 m a. s. l., 48 ° 09 ' 52 " N, 24 ° 17 ' 03 " E, 12. viii. 2021, Yu. Guglya (1 ♂); near Shyrokyi Luh, 48 ° 17 ' N, 24 ° 43 ' E, 19. vii. 2017, Yu. Guglya (1 ♂). Hosts. Apiaceae: Pimpinella L. (Benavent-Corai et al. 2015); Chaerophyllum aromaticum L — a newly recorded host plant. Wing. (Fig. 179) Hyaline, with beige veins; costa ending after R 3 + 4; second cross vein absent; calypterincluding margin pale beige, fringe beige. Wing length 2.0 mm. Female genitalia. (Figs. 180) Proctiger generally barrel-shaped, scarcely expanding posteriorly. Two pairs of long setae located on posterior margin between cerci. Cerci closely spaced. Cercus oval, 0.34 × as long as proctiger; medium setae located apically, subapically and laterally. Distribution. Austria, Germany, Great Britain, Hungary, Italy, Norway, Poland, Switzerland (Papp & Černý 2019). Ukraine (first record). Comments. See above under Phytomyza aurei. Mine, puparium and cephalopharingeal skeleton depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF36263CFF7BFE525B0232D2.taxon	materials_examined	Material examined. Ukraine: Ivano-Frankivsk Region: near Zarosliak: 1400 m a. s. l., 48 ° 10 ' 10 " N, 24 ° 31 ' 24 " E, 29. viii. 2022 — mine with larva collected, 31. viii. 2022 — pupation outside the mine, 12. ii. 2023 — imago, Yu. Guglya, ex Symphytum cordatum (1 ♂). Comments. Hosts, mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF36263CFF7BFC965AA834BE.taxon	materials_examined	Material examined. Ukraine: Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 06 ' E, 19. vi. 2022 — mines with puparia collected, 3 – 7. vii. 2022 — imago, Yu. Guglya, ex Mentha sp. (3 ♂ 3 ♀); same locality, 7. vi. 2020, Yu. Guglya (1 ♂); same locality, 20. vi. 2024 — mines with puparia collected, 30. vi. 2024 — imago, Yu. Guglya, ex Mentha spicata “ Crispa Marocco ” (1 ♂); Stara Pokrovka, 49 ° 48 ' N, 36 ° 32 ' E, 10. vi. 2022 — mines with puparia collected, 7. vii. 2022 — imago, N. Bludova, ex Mentha sp. (1 ♂). Hosts. Lamiaceae: Origanum vulgare L. (Spencer 1976), Mentha × piperita L., Mentha sp. (Guglya 2021), M. suaveolens Ehrh. (Eiseman & Lonsdale 2022). Mentha spicata L. — a newly recorded host plant. Comments. Mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF36263EFF7BFB7A5B96327E.taxon	description	(Figs. 81 – 84, 299 – 303) Material examined. Ukraine: Transcarpathia: Rakhiv, 430 m a. s. l., 48 ° 03 ' N, 24 ° 13 " E, 8. v. 2018 — mine collected, 11. v. 2018 — pupation outside the mine, Yu. Guglya, ex Aegopodium podagraria (1 ♂); Kyiv Region: Rzhyshchiv, 49 ° 58 ' 25 " N, 31 ° 06 ' 20 " E, 21. v. 2021 — mines collected, pupation outside the mine, x. 2021 – ii. 2022 — overwintered, 5 – 8. ii. 2022 — imago, Yu. Guglya, ex Aegopodium podagraria (2 ♀ 3 puparia); Kharkiv Region: Sharivka, 50 ° 01 ' 13 " N 35 ° 26 ' 52 " E, 10. v. 2016 — pupation outside the mine, Yu. Guglya, ex Aegopodium podagraria (1 ♂ 2 ♀). Host: Apiaceae: Aegopodium podagraria L. (Spencer 1976). Mine. (Fig. 81) The solitary larva forms a greenish or yellowish linear upper surface leaf mine, with grains and narrow lines of frass arranged irregularly along the margins irregularly; the third instar larva forms a very wide linear mine, that resembles a blotch. Pupation outside the mine. Puparium. (Figs. 82 – 84) Pale brown, glossy, 2.2 mm long, with distinct but shallow segmentation; surface quite smooth except the narrow bands of fine spines between all segments. Both pairs of spiracles black. Posterior spiracles set flat on the body cuticle and entirely separate; with 23 – 27 fine sessile bulbs in a kidney-shaped configuration. Two tiny sharp black appendages develope on each side of anal plate. Anal plate slightly protruding ventro-posteriorly viewed from the side. Cephalopharingeal skeleton. (Fig. 299 a, b) Right mouthhook larger dorsally and narrower than the left, both with ventro-anterior portion acute, abducted ventrally. Mouthhooks bear two sharp accessory teeth which are uniformly curved and directed ventrally. Mouthhooks and intermediate sclerite are strongly sclerotized, dorsal and ventral cornua much less so. Intermediate sclerite straight; bearing one sharp tooth on the posterior third of ventral margin directed ventrally. Ventral cornu bears long “ closed ” window posteriorly. Indentation index 84. Female genitalia. (Figs. 300 – 303) Spermathecae relatively large, 0.31 × as high as anterior part of oviscape. Proctiger generally cylindrical, slightly curve and concaved posteriorly; with two pairs medium setae located on posterior margin between cerci. Cerci distinctly spaced from each other. Cercus wide oval, flattened apically, 0.33 × as long as proctiger; medium and short setae located subapically and apically. Spermathecae equal in size, brown, with wrinkled surface, squared. Internal duct invagination cylindrical, 0.8 × as deep as height of spermatheca. Spermathecal duct narrow and very weakly sclerotized. Ventral receptacle brown, S-shaped, with well sclerotized tail that is bowl-shaped in basal half. Body of receptacle squared, with acutely curved basal connecting tube, strongly sclerotized. Opening large, equal to the diameter of spherical part of the body, located on wide bowl-shaped projection, 1.04 × as wide as a diameter of spherical part of the body. Basal connecting tube at the confluence of the tail with finger-shaped appendage, 2.0 × as wide as width of the tail. Distribution. West Palaearctic species recorded from 20 countries (Papp & Černý 2019). Ukraine (first record). Comments. There are 5 frorbs in all males and 1 female reared in Ukraine. Spencer (1976) and Papp & Černý (2019) noted 2 ors and 2 ori.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF34263EFF7BFD3A5AA833BA.taxon	materials_examined	Material examined. Ukraine: Kharkiv Region: Kharkiv, City Center, 50 ° 00 ' N, 36 ° 14 ' E, 15. vi. 2024 — numerous mines with larva and puparia collected, 25 – 30. vi. 2024 — imago, Yu. Guglya, ex Symphytum caucasicum x officinalis (9 ♂ 9 ♀). Comments. Mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF342624FF7BFC7E5844320A.taxon	description	(Figs. 85 – 93, 278 – 298) Material examined. Ukraine: Volyn Region: near Shatsk, 51 ° 28 ' 28 " N, 23 ° 48 ' 27 " E, 23. v. 2019, Yu. Guglya (2 ♀); near Pulmo, 51 ° 32 ' 07 " N, 23 ° 45 ' 49 " E, 24. v. 2019, Yu. Guglya (3 ♂); Transcarpathia: near Chop, 48 ° 27 ' N, 22 ° 12 ' E, 4. v. 2018, Yu. Guglya (1 ♀); near Vorochevo, 196 m a. s. l., 48 42 ’ N, 22 26 ’ E, 24. vi. 2018, Yu. Guglya (1 ♀); near Nevytske, 48 ° 41 ' N, 22 ° 25 ' E, 14. vii. 2017, Yu. Guglya (2 ♀); Kamianytsa, 48 ° 41 ' N, 22 ° 25 ' E, 07. v. 2018, Yu. Guglya (2 ♂ 2 ♀); same locality, 16. vii. 2017, Yu. Guglya (1 ♂); same locality, 18. vii. 2017 — pupation outside the mine, Yu. Guglya, ex Geranium sp. (1 puparium); between Antalivtsy and Nevytske, Antalivska Poliana, 936 m a. s. l., 48 ° 40 ' N, 22 ° 29 ' E, 05. v. 2018, Yu. Guglya (1 ♀); Uzhhorod, Kalvaria, 48 ° 37 ' N, 22 ° 17 ' E, 21. viii. 2017, Yu. Guglya (1 ♂); near Mukachevo, Karpaty, 48 ° 31 ' N, 22 ° 52 ' E, 18. viii. 2017, Yu. Guglya (2 ♂); Kvasy, 560 m a. s. l., 48 ° 9 ' 52 " N, 24 ° 17 ' 3 " E, 12. viii. 2021, Yu. Guglya (1 ♀); same locality, 14. viii. 2021 — pupation outside the mine, 30. viii. 2021 and 15. ii. 2022 — imago, Yu. Guglya, ex Geranium sp. (1 ♂ 1 ♀); same locality, 16. viii. 2021 — pupation outside the mine, 31. viii. – 1. ix. 2021 — imago, Yu. Guglya, ex Ranunculus sp. (3 ♀ 1 puparium); E Yasynia, 1105 m a. s. l., 48 12 ' 2 " N, 24 25 ' 9 " E, 20. viii. 2022 — pupation outside the mine, 1. v. 2023 — dried imago inside the puparium found, Yu. Guglya, ex Ranunculus repens (1 ♂ 1 ♀); 1 km N Rakhiv Center, 687 m a. s. l., 48 ° 2 ' N, 24 ° 15 ' E, 9. v. 2018, Yu. Guglya (1 ♀); Rakhiv, 650 m a. s. l., 48 ° 2 ' N, 24 ° 13 ' E, 22. vii. 2017, Yu. Guglya (1 ♀); 4 km S Rakhiv, 48 ° 1 ' N, 24 ° 10 ' E, 11. v. 2018, Yu. Guglya (1 ♀); near Lukh, Kuzyi location, 350 m a. s. l., 47 ° 56 ' N, 24 ° 6 ' E, 10. v. 2018, Yu. Guglya (1 ♀); between Hoverla and Petros Mts, “ Peremychka ”, 1530 m a. s. l., 48 09 ' 34 " N, 24 28 ' 06 " E, 19. viii. 2019 — pupation outside the mine, 5. ix. 2019 and 3. iii. 2020 — imago, Yu. Guglya, ex Ranunculus acris (2 ♀ 1 puparium); Ivano-Frankivsk Region: foothills of Dantsyzh Mt., 1460 m a. s. l., 48 8 ' 4 " N, 24 32 ' 52 " E, 28. viii. 2022 — pupation outside the mine, 16. ix. 2022 — imago, Yu. Guglya, ex Ranunculus sp. (1 ♂); Kyiv Region: near Rzhyshchiv, ecostation “ Hlyboki Balyky ”, 49 ° 57 ' 44 " N, 31 ° 07 ' 08 " E, 23. vi. 2021 — pupation outside the mine, 6. vii. 2021 — imago, Yu. Guglya, ex Ranunculus polyanthemos (5 ♂ 1 ♀); Kharkiv Region: N Kharkiv, 50 ° 4 ' N, 36 ° 18 ' E, 28. v. 2017, Yu. Guglya (1 ♀); near Petrivske, 49 ° 10 ' N, 36 ° 58 ' E, 17. vii. 2011 — pupation outside the mine, Yu. Guglya, ex Geranium sylvaticum (1 ♀); near Rubizhne, 50 ° 07 ' N, 36 ° 46 ' E, 21. v. 2017, Yu. Guglya (2 ♀); same locality, 19. vii. 2014, Yu. Guglya (1 ♂); near Haidary, 49 ° 37 ' N, 36 ° 19 ' E, 31. vii. 2020 — leaves with mines and puparia on lower leaf side outside the mine collected, 8. viii. 2020 — imago, Yu. Guglya, ex Geranium pratense (2 ♂). Phytomyza cf. ranunculivora: Transcarpathia: near Vorochevo, 196 m a. s. l., 48 42 ’ N, 22 26 ’ E, 24. vi. 2018, Yu. Guglya (1 ♂); Kharkiv Region: near Rubizhne, 50 10 ’ N, 36 47 ’ E, 21. vii. 2014 — pupation outside the mine, 2. viii. 2014 — imago, Yu. Guglya, ex Geranium palustre (1 ♂ 1 ♀). Hosts. Phytomyza ranunculivora: Ranunculaceae: Ranunculus L. (Benavent-Corai et al., 2005). Geraniaceae: Geranium pratense L. and G. sylvaticum L. — newly recorded host plants. Phytomyza cf. ranunculivora: Geraniaceae: Geranium palustre L. — a newly recorded host plant. Mine. Phytomyza ranunculivora (Fig. 85, 88): The solitary larva forms a white irregular linear upper surface leaf mine. Frass is arranged in large and small irregularly spaced grains. Pupation takes place outside the mine in the soil or attached to the lower leaf surface. Phytomyza cf. ranunculivora (Fig. 91): Mine shape and size are similar, with tunnels 1.5 × as wide as width of those in P. ranunculivora. Puparium. Phytomyza ranunculivora (Figs. 86, 87, 89, 90): Yellow, 1.8 mm long, with deep segmentation; surface quite smooth except last two abdominal segments finely wrinkled. Posterior spiracles set on wide and short conical protuberances that are entirely separate; blackish-brown, glossy and two-horned. Ventral portion of last abdominal segment distinctly protruding posteriorly viewed from the side. Anal plate yellow or dark yellow, distinctly protruding above the surface of the puparium viewed from the side and directed ventro-posteriorly. Phytomyza cf. ranunculivora (Figs. 92, 93): The puparium is uniformly paler than in P. ranunculivora. Cephalopharingeal skeleton. Phytomyza ranunculivora (Fig. 281): Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventrally. Mouthhooks bear two sharp accessory teeth which are uniformly curved and directed ventrally; dorsal teeth slightly larger than ventral. Right mouthhook bears elongated more weakly sclerotized sclerite dorsally. Mouthhooks, intermediate sclerite and anterior two-fifths of dorsal cornu are strongly sclerotized. Intermediate sclerite straight, uniformly tapering anteriorly, bearing two sharp teeth — on ventral margin directed ventrally and on dorsal margin directed dorso-anteriorly. Well sclerotized part of dorsal cornu 0.19 × as wide as height of right mouthhook. Ventral cornu bears large “ closed ” window posteriorly. Indentation index 80. Phytomyza cf. ranunculivora (Fig. 288): Both mouthhooks with ventro-anterior portion rounded, abducted ventrally. Right mouthhook bears triangular more weakly sclerotized sclerite dorsally. Intermediate sclerite bears one sharp tooth on the posterior third of ventral margin directed ventro-posteriorly. Well sclerotized part of dorsal cornu 0.12 × as wide as height of right mouthhook. Indentation index 77. Female head. Phytomyza ranunculivora (Figs. 278, 279): Bicolour — frons, lunule and gena yellowish-brown, the rest blackish-brown; 1 st fl of medium size, elongate, with thick pale pubescence; fronto-orbital plate not visible laterally, shining, with distinct contours (frontal view); 1 ors, 1 – 2 ori, lower very short; lunule of medium height, broad, rounded dorsally, reaching the level of ori p; gena and parafacial together medially 0.2 × as high as maximum vertical height of eye. Phytomyza cf. ranunculivora (Figs. 285, 286): Bicolour — frons, lunule, parafacial and gena pale brown, the remaining brown; fronto-orbital plate not shining, colour of fronto-orbital plate and frons slightly differ; 2 ors, 1 ori. Wing. Phytomyza ranunculivora (Fig. 280): Hyaline, with yellowish undertone; with costa, R 1 + 2, R 3 + 4 brown, the remaining veins pale; costa ending after R 3 + 4; second cross vein absent; calypter and margin beige, fringe slightly darker. Wing length 2.0 mm. Phytomyza cf. ranunculivora (Fig. 287): As in P. ranunculivora. Male genitalia. Phytomyza ranunculivora (Figs. 293, 295): Postgonite large, elongated, 3.6 × as long as its maximum width; widest in apical half; bears one long seta; with set at a distance finger-shaped uniformly curved lobe located apically. Hypophallus arcuate viewed from below, strongly sclerotized, 0.9 × as long as basiphallus viewed from the side. Male terminalia except postgonite depicted and described in Papp & Černý (2019). Phytomyza cf. ranunculivora (Figs. 292, 294, 296 – 298): Hypandrium U-shaped, with arms widening anteriorly. Postgonite large, elongated, 3.35 × as long as its maximum width; widest medially; bears one long seta; with slightly set at a distance acute lobe located apically. Ejaculatory apodeme small, with triangular blade; base small, curved, with sperm sac 0.4 × as wide as apodemal blade. Hypophallus arcuate viewed from below, relatively shorter and less sclerotized than in P. ranunculivora, 0.7 – 0.8 × as long as basiphallus viewed from the side. Tubules of distiphallus arched in medial third, directed anteriorly in reared specimen and dorso-anteriorly in captured specimen viewed from the side. Basiphallus, paramesophalli and basal third of distophallus similar to those in P. ranunculivora (see in Papp & Černý 2019). Female genitalia. Phytomyza ranunculivora (Figs. 282 – 285): Spermathecae of medium size, 0.21 – 0.27 × as high as anterior part of oviscape. Proctiger generally cylindrical, slightly rounded anteriorly and posteriorly; with pair of long setae located on posterior margin between cerci. Cerci located not far from each other, distance between them 0.4 × as wide as cercus. Cercus angulate, oblique apically, 0.24 × as long as proctiger; short setae located apically, few long setae located subapically. Spermathecae unequal in size, brown, with wrinkled surface, squared, slightly tapering apically. Internal duct invagination tapering medially, as deep as height of spermatheca. Spermathecal duct narrow, wrinkled, partly with distinct sclerotization; length of more sclerotized portion of spermathecal duct 6.66 × as long as height of spermatheca. Ventral receptacle brown, S-shaped, with well sclerotized tail that is bowl-shaped in basal half. Body of receptacle oval, wider than high, 1.52 × as wide as its height; with slightly curved basal connecting tube, as long as width of oval part, strongly sclerotized. Opening of medium size, 0.7 × as wide as the diameter of oval part of the body. Basal connecting tube 1.4 × as wide as tail. Phytomyza cf. ranunculivora (Figs. 289 – 291): Spermathecae of medium size, 0.24 × as high as anterior part of oviscape. Proctiger uniformly widened posteriorly; with four pairs of medium setae located on posterior margin between cerci. Cerci spaced from each other, distance between them as wide as width of cercus. Cercus 0.2 × as long as proctiger; few medium and long setae located apically and subapically. Spermathecae equal in size, brown, wider than high, distinctly tapering apically. Internal duct invagination 0.8 × as deep as height of spermatheca. Spermathecal duct very weakly sclerotized. Ventral receptacle brown, S-shaped, with well sclerotized tail that is bowl-shaped in basal half. Body of receptacle 1.4 × as wide as its height; with slightly curved basal connecting tube, 1.35 × as long as width of oval part, strongly sclerotized. Opening 0.6 × as wide as the diameter of oval part of the body. Basal connecting tube, 2.0 × as wide as tail. Distribution. European species recorded from 22 countries (Papp & Černý 2019). Ukraine (first record).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF342624FF7BFC7E5844320A.taxon	discussion	Comments. The earliest specimen in Ukraine was captured at the beginning of May and the last mines with larvae were observed at the end of August. Three generations were registered in Ukraine (Table 1). During 2011 – 2022 in five sites of Ukraine, males and females of P. ranunculivora from Geranium sp., G. palustre, G. pratense and G. sylvaticum were reared. Leaves of buttercup and geranium can be similar, but all mines were collected during flowering or fruiting of hosts which eliminates the possibility of misidentifying Ranunculus as Geranium. One captured male and a male and female reared from Geranium palustre, differ in many details from P. ranunculivora, as indicated above under P. cf. ranunculivora. Ellis (2020) refers to mines of unknown agromyzid species in Geranium sp. leaves from the Netherlands that resembles mines of Phytomyza ranunculivora and P. cf. ranunculivora collected in Ukraine. Several species are known from P. hendeli group (Winkler et al. 2009), including the European species P. ranunculivora Hering and P. lingua Lundquist feed on Ranunculus spp. and Geranium spp. and Ranunculus lingua L. respectively (Ranunculaceae, Geraniaceae) (Pakalniškis 1996, current data), P. rectae Hendel which feeds on Clematis recta L. (Ranunculaceae), P. albimargo Hering, P. hendeli Hering and P. pulsatilla Hering miners of Anemone species (Ranunculaceae), P. brischkei Hendel miners of Anthyllia vulneraria L. (Fabaceae), P. sedicola Hering a leaf miner of Hylotelephium spp. (= Sedum) (Crassulaceae) (Spencer 1976, Papp & Černý 2017), the Oriental P. rubicola Sasakawa which feeds inside leaves of Rubus palmatus (Rosaceae) (Sasakawa & Matsumura 1998), the Nearctic species P. multifida Sehgal which feeds on Anemone multifida Poir and A. quinquefolia L. (Eiseman et al 2015) and P. thalictrella Spencer (Ranunculaceae) which feeds on Thalictrum fendleri Engelm. ex A. Gray (Spencer 1981). Additionally, Wrinkler et al. (2009) suggested that the rare European species P. lappivora Hendel which feeds on Arctium tomentosum L. (Asteraceae) (Hendel 1924) belongs to the hendeli group and didn’t include the oriental Clematis- miner P. esakii Sasakawa (Sasakawa 1961) in any group, but suggested that it is from aquilegiae clade. In accordance with the structure of the male genitalia (Spencer 1971, Sasakawa 1961) they both are clearly from the hendeli group. This group of species demonstrates a wide range of host specialization — plants of nine genera from six families.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF2E2624FF7BFDEF5B023361.taxon	materials_examined	Material examined. Ukraine: Ivano-Frankivsk Region: near Zarosliak: 1400 m a. s. l., 48 ° 10 ' 10 " N, 24 ° 31 ' 24 " E, 17. viii. 2021 — mines with larva collected, 19 – 21. viii. 2021 — pupation outside the mine, Yu. Guglya, ex Senecio nemorensis (1 ♂ 3 puparia); 1460 m a. s. l., 48 ° 08 ' 04 " N, 24 ° 32 ' 52 " E, 25. viii. 2022 — mins with larva collected, 29. viii. 2022 — pupation outside the mine, Yu. Guglya, ex Senecio nemorensis (2 puparia). Comments. Hosts, mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF2E2621FF7BFC475BA1352E.taxon	description	(Figs. 94 – 99, 304 – 318) Holotype (KUMN). “ Ukraine, Kharkiv, City / Center, 50 ° 00 ' N 36 ° 14 ' E / 23. iv. 2020 — collected / 10. xii. 2020 — imago / Yu. Guglya leg. ”, “ narrow blotch mine / along the midrib on / Taraxacum officinale, / 28. iv. 2020 — pupation / outside the mine (1 ♂). Paratypes (KUMN). “ Ukraine, Kharkiv, City Center, / 50 ° 00 ' N 36 ° 14 ' E, / 23. iv. 2020 — collected / 11. xii. 20 — developed / imago inside the puparium ”, “ white blotch / gregarious mine on / Lactuca serriola, / 25 - 30. iv — pupar. outside / Yu. Guglya leg. ” (3 ♂ 3 ♀); “ Ukraine, Kharkiv Region / near Petrivske / 49 ° 10 ' N 36 ° 58 ' E / 2. v. 2020 / Yu. Guglya leg. ”, “ white blotch / mine on Lactuca serriola / 3 - 4. v. 2020 — pupation / outside, 8. xi. 2020 — / developed fly inside pup. ” (1 ♂ 2 ♀); “ Ukraine, Vinnytsa / Region, near Chechelnyk, / Vyshenka location / 48 ° 10 ' 45 " N 29 ° 20 ' 00 " E / 8. v. 2019 Yu. Guglya leg. ”, “ blotch mine with larva / on Lactuca serriola / 11. v. 2019 — 7 puparia / outside the mine, dried / developed fly inside pup. ” (1 ♂). In addition collection consists of 13 puparia with not developed flies from all three localities. Etymology. The name of the new species reflects its host specialization. Most of the larvae were collected in Lactuca serriola leaves. Hosts. Asteraceae: Lactuca serriola L., Tarxacum officinale F. H. Wigg. Mine. (Fig. 94, 95) The gregarious larvae form a white upper surface leaf mines located mainly along the midrib. Pupation tales place either within or outside the mine in a soil. Puparium. (Figs. 96 – 99) Bright yellow, 3.0 mm long, with distinct segmentation; surface wrinkled, more between segments; a row of sparse large spines encircles each segment medially. All spiracles are blackish-orange. Posterior spiracles set on short separated cylindrical protuberances; with 22 – 24 minute spherical sessile bulbs in a 8 - shaped configuration. Two small dark orange appendages developed between anal plate and posterior spiracles well seen from the side. Anal plate yellow, anus blackish-orange; directed ventro-posteriorly. Cephalopharingeal skeleton. (Fig. 309) Right mouthhook larger dorsally than the left, both with ventro-anterior portion acute, abducted ventrally. Mouthhooks bear two sharp accessory teeth which are uniformly curved and directed ventrally; dorsal teeth larger than ventral. Mouthhooks and anterior half of intermediate sclerite are strongly sclerotized, posterior half of intermedial sclerite, dorsal and ventral cornua much less so. Intermediate sclerite straight, cylindrical; bears one sharp tooth on the posterior third of ventral margin that is directed ventrally. Ventral cornu bears small “ closed ” window posteriorly. Indentation index 86. Distribution. Ukraine (Kharkiv and Vinnytsa Regions). Adult description. Head (Figs. 304 – 306) dark yellow, with occiput, post gena, palpi brown, 1 st fl and ocellar tubercle black. Fronto-orbital plate distinctly visible laterally; 2 ors, 2 ori, with areolae brown, arranged in flat arc configuration viewed from above (Fig. 305); orbital setula in 3 – 4 irregular rows, small, sparse, proclinate; i vt s and o vt s in brown ground; fronto-orbital plate wide; parafacial extremely wide; lunule of medium size, reaching the level of the ori p; gena and parafacial together medially 0.58 × as wide as maximum height of eye; gena wider at rear; 1 st fl elongated, rounded apically, covered with thick pale hairs clearly viewed from the side. Wing: (Fig. 307) Slightly greyish, with brownish veins; costa ending after R 3 + 4; second cross vein absent; calypter and margin yellowish-brown, fringe black. Wing length 2.0 mm. Mesonotum: (Fig. 308) Ash grey, silky-shining viewed from above, blackish-brown viewed from the side, same as sctl; 1 + 3 dc s, posterior pair slightly longer than others; acr s absent; halter yellow; legs blackish-brown with all knees dark orange. Male genitalia. (Figs. 313 – 318) Epandrium subovate; cerci small and narrow, 0.3 × as long as height of epandrium in posterior view; hypandrium narrow U-shaped, with narrow arms, pregonite wider than long, bearing weakly sclerotized narrow lobe anteriorly; located on the arm medially, bearing 0 – 2 small setae near the inner basal corner viewed from below. Postgonite large, elongated, 2.35 × as long as its maximum width, with closely spaced triangular lobe located apically. Ejaculatory apodeme small; narrow blade with unevently torn apical edge; base with sperm sac 0.9 × as wide as apodemal blade. Basiphallus and hypophallus strongly sclerotized, distiphallus is weakly so. Basiphallus long, straight, bearing large acute posteriorly widening lobe, which is more weakly sclerotized. Hypophallus consists of one fractured ventro-anteriorly directed sclerite, which is slightly widening apically viewed from the side and acute viewed from below. Mesophallus and paraphallus not evident. Distiphallus long, bearing well sclerotized beak-shaped appendage apically; scarcely sclerotized tubule-shaped part with two long lateral strongly sclerotized areas located basally. Posterior part of distiphallus cup-shaped, wrinkled. Female genitalia. (Figs. 310 – 312) Proctiger wide in posterior three quarters; dramatically narrowing anteriorly; two pairs of long setae located on the posterior margin between cerci. Cercus wide, oval, tapering apically, 0.35 × as long as proctiger; short and medium setae located apically, subapically and interna-laterally. Ventral receptacle brown, S-shaped, with well sclerotized tail that bears weakly sclerotized lobes in basal half; these lobes narrow basally and widening and rounded apically. Body of receptacle spherical, with slightly curved basal connecting tube, strongly sclerotized. Opening small, 0.44 × as wide as a diameter of spherical part of the body, located on wide projection that is ring-shaped basally and beak-shaped apically. Basal connecting tube at the confluence of the tail 3.33 × as wide as tail. Comments. Mines with larvae of this species can be collected only in mid-spring (late April to early May). One generation develops per year.	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
5135879CBF2B2621FF7BFA0A5B02364E.taxon	materials_examined	Material examined. Ukraine: Kharkiv Region: N Kharkiv, Piatykhatky, 50 ° 05 ' 42 " N 36 ° 15 ' 38 " E, 10. vii. 2021 — mine with puparium collected, 15. x. 2021 — dead imago found, Yu. Guglya, ex Cirsium arvense (1 ♀). Comments. Hosts, mine, puparium, cephalopharingeal skeleton and female genitalia depicted and described in Guglya (2021).	en	Guglya, Yuliia (2025): Rearing mining flies (Diptera: Agromyzidae) from host plants as an instrument for associating females with males, with the description of two new species. Report 2. Zootaxa 5658 (1): 1-86, DOI: 10.11646/zootaxa.5658.1.1, URL: https://doi.org/10.11646/zootaxa.5658.1.1
